Anti-TXNDC5抗体(ab10292)
Key features and details
- Rabbit polyclonal to TXNDC5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TXNDC5抗体
参阅全部 TXNDC5 一抗 -
描述
兔多克隆抗体to TXNDC5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Mouse TXNDC5 aa 50 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q91W90 -
阳性对照
- WB: HepG2, HeLa and HEK293 whole cell lysate.
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常规说明
ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10292于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
靶标
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功能
Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast. -
序列相似性
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 81567 Human
- SwissProt: Q8NBS9 Human
- Unigene: 150837 Human
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别名
- EndoPDI antibody
- Endoplasmic reticulum protein ERp46 antibody
- Endoplasmic reticulum resident protein 46 antibody
see all
图片
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Western blot - Anti-TXNDC5 antibody (ab10292)All lanes : Anti-TXNDC5 antibody (ab10292) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 60 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab10292 被引用在 3 文献中.
- Pérez-Vargas J et al. A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process. Elife 10:N/A (2021). PubMed: 34190687
- Alberti A et al. ERp46 is reduced by high glucose and regulates insulin content in pancreatic beta-cells. Am J Physiol Endocrinol Metab 297:E812-21 (2009). WB, ICC/IF ; Mouse . PubMed: 19622788
- Knoblach B et al. ERp19 and ERp46, New Members of the Thioredoxin Family of Endoplasmic Reticulum Proteins. Mol Cell Proteomics 2:1104-19 (2003). PubMed: 12930873