AB249787

重组Anti-TXNDC13/TMX4抗体[EPR12321] - BSA and Azide free

Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TXNDC13/TMX4 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

查看别名

KIAA1162, TXNDC13, PSEC0095, UNQ475/PRO938, TMX4, Thioredoxin-related transmembrane protein 4, Thioredoxin domain-containing protein 13

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free (AB249787)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free (AB249787)

This data was developed using ab174276, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A375 cells labeling TXNDC13/TMX4 with ab174276 at 1/50 dilution (red). DAPI nuclear staining (blue).

Supplier Data

Immunoprecipitation - Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free (AB249787)

This data was developed using ab174276, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from (1) A375 cell lysate or (2) 1X PBS (negative control) using ab174276 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TXNDC13/TMX4 antibody [EPR12321] (<a href='/products/primary-antibodies/txndc13-tmx4-antibody-epr12321-ab174276'>ab174276</a>)

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-TXNDC13/TMX4 antibody [EPR12321] - BSA and Azide free (AB249787)

This data was developed using ab174276, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TXNDC13/TMX4 antibody [EPR12321] (<a href='/products/primary-antibodies/txndc13-tmx4-antibody-epr12321-ab174276'>ab174276</a>) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Predicted band size: 39 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-TXNDC13/TMX4 antibody [EPR12321]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12321

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab249787 is the carrier-free version of ab174276.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC13 also known as TMX4 is a protein involved in redox reactions functioning as a thioredoxin-like enzyme. This protein has a molecular mass of approximately 45 kDa and is located primarily in the endoplasmic reticulum. Expression of TXNDC13/TMX4 is observed in various tissues but it is notably high in ones where protein folding and secretion activities are extensive. The involvement of TXNDC13/TMX4 in protein disulfide isomerization centers around its ability to catalyze the formation and breaking of disulfide bonds in proteins which is essential for proper protein folding and maturation.

Biological function summary

The role of thioredoxin-like proteins such as TXNDC13/TMX4 is critical in maintaining cellular redox balance and participating in oxidative stress response. TXNDC13/TMX4 does not appear to be a part of a larger protein complex but operates by interacting directly with other proteins as a chaperone. It helps in the formation of disulfide bonds and alleviates misfolded proteins ensuring correct protein structure and function. This activity is particularly significant in cells with high secretory demands.

Pathways

TXNDC13/TMX4 is involved in pathways governing cellular redox homeostasis and the unfolded protein response. It acts in the thioredoxin pathway an important regulator of the cellular redox environment. The protein's functioning relates to other proteins such as PDI which are key elements in the protein-folding machinery within the endoplasmic reticulum. TXNDC13/TMX4 works closely with such proteins to manage oxidation and reduction reactions essential for controlling cellular oxidative stress levels.

TXNDC13/TMX4 links with conditions involving oxidative stress and protein misfolding such as neurodegenerative diseases and certain cancers. Dysregulation in this protein's activity can contribute to the aggregation of misfolded proteins exacerbating disorders like neurodegeneration. It may also have a role in tumor biology where altered redox states are often observed. The protein is connected to others like TXN which also influence cellular responses to oxidative stress further implicating it in the pathological mechanisms of these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information TMX4

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重组Anti-TXNDC13/TMX4抗体[EPR12321] - BSA and Azide free