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Anti-Twist抗体[10E4E6] (ab175430)

Reviews (3) Submit a question References (88)
Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)

Key features and details

  • Mouse monoclonal [10E4E6] to Twist
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Twist抗体[10E4E6]
    参阅全部 Twist 一抗

  • 描述

    小鼠单克隆抗体[10E4E6] to Twist

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, IHC-P, WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human, Recombinant fragment
    预测可用于: Chimpanzee, Gorilla, Common marmoset

  • 免疫原

    Recombinant fragment corresponding to Human Twist aa 9-74. (Expressed in E.coli).
    Sequence:

    PVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGG GVGGGDEPGSPAQGKR


    Database link: Q15672
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Twist recombinant protein; Twist (aa9-74) - hIgGFc transfected Hek293 cell lysate; NIH3T3, Jurkat, HeLa, A549, Raji and OCM-1 cell lysates; HeLa cells; cervical cancer tissue; colon cancer tissue.

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR313044-1 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175430于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
WB (1)
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.
ICC/IF
1/200 - 1/1000.
说明
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
WB
1/500 - 1/2000. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA as the blocking agent.
ICC/IF
1/200 - 1/1000.

靶标

  • 功能

    Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.

  • 组织特异性

    Subset of mesodermal cells.

  • 疾病相关

    Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.

  • 序列相似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS antibody
    • CRS1 antibody
    • CSO antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist family bHLH transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

图片

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Raji cell lysate
    Lane 6 : OCM-1 cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)
    Immunocytochemistry/ Immunofluorescence - Anti-Twist antibody [10E4E6] (ab175430)

    Immunofluorescent analysis of Hela cells labeling Twist with ab175430 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)
    Flow Cytometry - Anti-Twist antibody [10E4E6] (ab175430)

    Flow cytometric analysis of HeLa cells labeling Twist with ab175430 at 1/200 dilution (green) compared to a negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Twist antibody [10E4E6] (ab175430)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling Twist with ab175430 at 1/200 dilution, with DAB staining.

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    All lanes : Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Twist (aa9-74) - hIgGFc transfected HEK293 cell lysate

    Predicted band size: 21 kDa

  • Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Western blot - Anti-Twist antibody [10E4E6] (ab175430)
    Anti-Twist antibody [10E4E6] (ab175430) at 1/500 dilution + Twist recombinant protein

    Predicted band size: 21 kDa



    Expected MW is 31.9 kDa.

文献 (88)

发表研究结果有使用 ab175430？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab175430 被引用在 88 文献中.

  • Zhang X  et al. Over-Expression of ARID3B Suppresses Tumor Progression and Predicts Better Prognosis in Patients With Gastric Cancer. Cancer Control 30:10732748231169403 (2023). PubMed: 37071790
  • Xu X  et al. TPPP3 promote epithelial-mesenchymal transition via Snail1 in glioblastoma. Sci Rep 13:17960 (2023). PubMed: 37863960
  • Wang YL  et al. Sestrin2 mediates FOXM1 expression to block the EMT process in non-small cell lung cancer through the AMPK/YAP pathway. Neoplasma 70:46-57 (2023). PubMed: 36620877
  • Chen SN  et al. E2F2 modulates cell adhesion through the transcriptional regulation of PECAM1 in multiple myeloma. Br J Haematol 202:840-855 (2023). PubMed: 37365680
  • Liang X  et al. Inhibition of lncRNA RET enhances radio-sensitivity of tumor cells via miR-3179/Slug/PTEN axis. Toxicol Res (Camb) 11:348-360 (2022). PubMed: 35510230
View all Publications for this product

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Western blot abreview for Anti-Twist [10E4E6] antibody

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 May 20 2014

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