重组Anti-TWEAKR/FN14抗体[EPR3179] (ab109365)
![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.cn/ps/products/109/ab109365/Images/ab109365-266756-anti-tweakr-antibody-epr3179-western-blot.jpg "Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3179] to TWEAKR/FN14
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TWEAKR/FN14抗体[EPR3179]
参阅全部 TWEAKR/FN14 一抗 -
描述
兔单克隆抗体[EPR3179] to TWEAKR/FN14 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3179 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109365于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
|
|
| IP |
1/40.
For unpurified use at 1/10. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa. |
|
IP
1/40. For unpurified use at 1/10. |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. -
组织特异性
Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas. -
序列相似性
Contains 1 TNFR-Cys repeat. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 51330 Human
- Entrez Gene: 27279 Mouse
- Entrez Gene: 302965 Rat
- Omim: 605914 Human
- SwissProt: Q9NP84 Human
- SwissProt: Q9CR75 Mouse
- Unigene: 355899 Human
- Unigene: 28518 Mouse
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别名
- CD 266 antibody
- CD266 antibody
- CD266 antigen antibody
see all
图片
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Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM /TBST -
![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.cn/ps/products/109/ab109365/Images/ab109365-266757-anti-tweakr-antibody-epr3179-western-blot.jpg)
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : L6 (rat skeletal muscle) whole cell lysate
Lane 3 : C2C12 (mouse muscle) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14-17 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer 5% NFDM /TBST -
![Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.cn/ps/products/109/ab109365/Images/ab109365-266799-anti-tweakr-antibody-epr3179-immunoprecipitation.jpg)
Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)ab109365 at 1/40 immunoprecipitating TWEAKR/FN14 in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).
Lane 1 (input): HUVEC whole cell lysate 10ug
Lane 2 (+): ab109365 + HUVEC whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.cn/ps/products/109/ab109365/Images/ab109365-122129-anti-tweakr-antibody-epr3179-western-blot.jpg)
Western blot - Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)All lanes : Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365) at 1/1000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 14 kDa -
![Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)](https://www.abcam.cn/ps/products/109/ab109365/Images/ab109365-8-benefits-of-recombinant-antibodies.png)
Anti-TWEAKR/FN14 antibody [EPR3179] (ab109365)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab109365 被引用在 24 文献中.
- Huang S et al. LINC02381 aggravates breast cancer through the miR-1271-5p/FN1 axis to activate PI3K/AKT pathway. Mol Carcinog 61:346-358 (2022). PubMed: 34882856
- Wolf J et al. Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration. Biochim Biophys Acta Mol Basis Dis 1868:166340 (2022). PubMed: 35032596
- Sommer A et al. Antibody-drug conjugates harboring a kinesin spindle protein inhibitor with immunostimulatory properties. Oncoimmunology 11:2037216 (2022). PubMed: 35154909
- Wang GY et al. Nrf2 deficiency causes hepatocyte dedifferentiation and reduced albumin production in an experimental extrahepatic cholestasis model. PLoS One 17:e0269383 (2022). PubMed: 35696363
- Li Z et al. Inhibition of TWEAK/Tnfrsf12a axis protects against acute liver failure by suppressing RIPK1-dependent apoptosis. Cell Death Discov 8:328 (2022). PubMed: 35853848