Anti-Tubulin抗体[4D1] - Loading Control (ab56676)
![Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)](https://www.abcam.cn/ps/products/56/ab56676/Images/ab56676-70076-anti-tubulin-antibody-loading-control-western-blot.jpg "Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)")
Key features and details
- Mouse monoclonal [4D1] to Tubulin - Loading Control
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-Tubulin抗体[4D1] - Loading Control
参阅全部 Tubulin 一抗 -
描述
小鼠单克隆抗体[4D1] to Tubulin - Loading Control -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Tubulin aa 1-451.
Database link: P68363 -
常规说明
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
4D1 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56676于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
|
| IHC-P | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 10376 Human
- Entrez Gene: 203068 Human
- Entrez Gene: 7280 Human
- Omim: 191130 Human
- Omim: 602530 Human
- Omim: 615101 Human
- SwissProt: P07437 Human
- SwissProt: P68363 Human
see all -
别名
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
see all
图片
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Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)
Tubulin antibody (ab56676) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)](https://www.abcam.cn/ps/products/56/ab56676/Images/ab56676-70077-anti-tubulin-antibody-loading-control-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)Tubulin antibody (ab56676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)](https://www.abcam.cn/ps/products/56/ab56676/Images/ab56676-70074-anti-tubulin-antibody-loading-control-western-blot.jpg)
Western blot - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)This image is courtesy of an Abreview submitted by Xun AiAll lanes : Anti-Tubulin antibody [4D1] - Loading Control (ab56676) at 1 µg/ml (in TPBS for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of rabbit heart at 20 µg
Lane 2 : Whole tissue lysate of rabbit heart at 40 µg
Lane 3 : Whole tissue lysate of rabbit heart at 60 µg
Lane 4 : Whole tissue lysate of rabbit heart at 80 µg
Lane 5 : Whole tissue lysate of rabbit heart at 100 µg
Lane 6 : Whole tissue lysate of rabbit heart at 120 µg
Secondary
All lanes : An HRP-conjugated Donkey anti-mouse IgG polyclonal at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking Step : 5% BSA for 2 hours at 23°C
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)](https://www.abcam.cn/ps/products/56/ab56676/Images/ab56676-70073-anti-tubulin-antibody-loading-control-flow-cytometry.jpg)
Flow Cytometry - Anti-Tubulin antibody [4D1] - Loading Control (ab56676)Overlay histogram showing HeLa cells stained with ab56676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56676, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab56676 被引用在 42 文献中.
- Cao Q et al. A single-domain i-body, AD-114, attenuates renal fibrosis through blockade of CXCR4. JCI Insight 7:N/A (2022). PubMed: 35015734
- Trombetta-Lima M et al. Impact of Reck expression and promoter activity in neuronal in vitro differentiation. Mol Biol Rep 48:1985-1994 (2021). PubMed: 33619662
- Han J et al. Improved lentiviral vector titers from a multi-gene knockout packaging line. Mol Ther Oncolytics 23:582-592 (2021). PubMed: 34938858
- Canu V et al. Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation. Cell Death Differ 28:1493-1511 (2021). PubMed: 33230261
- Hwang S et al. Modulated Start-Up Mode of Cancer Cell Migration Through Spinophilin-Tubular Networks. Front Cell Dev Biol 9:652791 (2021). PubMed: 33768098