重组Anti-Tuberin (phospho S664)抗体[EPR8202] (ab133465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8202] to Tuberin (phospho S664)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tuberin (phospho S664)抗体[EPR8202]
参阅全部 Tuberin 一抗 -
描述
兔单克隆抗体[EPR8202] to Tuberin (phospho S664) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y cell lysates treated with Okadaic acid and Calyculin A and HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled, Human, mouse and rat brain tissue lysate unboiled
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.12 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8202 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 201 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 201 kDa. |
靶标
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功能
In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. -
组织特异性
Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta. -
疾病相关
Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex. -
序列相似性
Contains 1 Rap-GAP domain. -
翻译后修饰
Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. -
细胞定位
Cytoplasm. Membrane. At steady state found in association with membranes. - Information by UniProt
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数据库链接
- Entrez Gene: 7249 Human
- Entrez Gene: 22084 Mouse
- Entrez Gene: 24855 Rat
- Omim: 191092 Human
- SwissProt: P49815 Human
- SwissProt: Q61037 Mouse
- SwissProt: P49816 Rat
- Unigene: 90303 Human
see all -
别名
- FLJ43106 antibody
- LAM antibody
- OTTHUMP00000158940 antibody
see all
图片
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Mouse brain lysate boiled
Lane 2 : Mouse brain lysate unboiled
Lane 3 : Rat brain lysate boiled
Lane 4 : Rat brain lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
We recommend not to boil the samples after lysis to get desired WB results.
The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Human brain lysate unboiled
Lane 2 : Human brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 3 : Rat brain lysate unboiled
Lane 4 : Rat brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 5 : Mouse brain lysate unboiled
Lane 6 : Mouse brain lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration 5% NFDM/TBST
Exposure time:
Lane 1, 2: 180 seconds
Lane 3, 4: 40 seconds
Lane 5, 6: 20 seconds -
All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate unboiled
Lane 2 : HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled
Lane 3 : HeLa treated with 200nM Phorbol-12-myristate-13-acetate (PMA) for 4 hours whole cell lysate unboiled, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 201 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Exposure time: 80 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
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All lanes : Anti-Tuberin (phospho S664) antibody [EPR8202] (ab133465) at 1/1000 dilution
Lane 1 : SH SY5Y cell lysates
Lane 2 : SH SY5Y cell lysates treated with Okadaic acid and Calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 201 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab133465 被引用在 5 文献中.
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362
- Plescher M et al. TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1. Sci Rep 5:13828 (2015). PubMed: 26345496
- Zhang Y et al. PP2AC Level Determines Differential Programming of p38-TSC-mTOR Signaling and Therapeutic Response to p38-Targeted Therapy in Colorectal Cancer. EBioMedicine 2:1944-56 (2015). PubMed: 26844273
- Peruchetti DB et al. Mis-regulation of Mammalian Target of Rapamycin (mTOR) Complexes Induced by Albuminuria in Proximal Tubules. J Biol Chem 289:16790-801 (2014). WB . PubMed: 24790108
- Jeansonne D et al. Differential Effects of MicroRNAs on Glioblastoma Growth and Migration. Genes (Basel) 4:46-64 (2013). WB . PubMed: 24705102