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重组RabMAb

重组Anti-TUBA4A抗体[EPR13477(B)] (ab177479)

Submit a review Submit a question References (1)
Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Flow Cytometry (Intracellular) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
  • Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13477(B)] to TUBA4A
  • Suitable for: IP, IHC-P, WB, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

概述

  • 产品名称

    Anti-TUBA4A抗体[EPR13477(B)]
    参阅全部 TUBA4A 一抗

  • 描述

    兔单克隆抗体[EPR13477(B)] to TUBA4A

  • 宿主

    Rabbit

  • 特异性

    Tubulin proteins have very high homology. The immunogen for this product is based on the TUBA4A protein. There is a chance that this product will detect other tubulin family members. The cross reactivity has not been experimentally determined.

  • 经测试应用

    适用于: IP, IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human TUBA4A aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P68366

  • 阳性对照

    • Molt-4, A431, Jurkat, HeLa and K562 cell lysates; Human testis and Human uterus tissues; A431 cells; K562 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR13477(B)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab177479于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
IP
1/10 - 1/100.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 50 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

  • 序列相似性

    Belongs to the tubulin family.

  • 翻译后修饰

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 alpha antibody
    • TBA4A_HUMAN antibody
    • Testis specific alpha tubulin antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA 4A antibody
    • TUBA1 antibody
    • Tuba4a antibody
    • Tubulin alpha 1 (testis specific) antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 4a antibody
    • Tubulin alpha 4A chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin H2-alpha antibody
    see all

图片

  • Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Western blot - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    All lanes : Anti-TUBA4A antibody [EPR13477(B)] (ab177479) at 1/1000 dilution

    Lane 1 : Molt-4 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 50 kDa

  • Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunoprecipitation - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab177479, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TUBA4A with ab177479 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Immunocytochemistry/ Immunofluorescence - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Immunofluorescent analysis of A431 cells labeling TUBA4A with ab177479 at 1/100 dilution (red). DAPI nuclear staining (blue).

  • Flow Cytometry (Intracellular) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Flow Cytometry (Intracellular) - Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling TUBA4A with ab177479 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). 

     

     

  • Anti-TUBA4A antibody [EPR13477(B)] (ab177479)
    Anti-TUBA4A antibody [EPR13477(B)] (ab177479)

文献 (1)

发表研究结果有使用 ab177479？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab177479 被引用在 1 文献中.

  • Hausrat TJ  et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development. Dev Neurobiol 81:333-350 (2021). PubMed: 32293117

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