重组TUBA1B抗体[EPR1333](ab108629)|Abcam中文官网

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-TUBA1B antibody [EPR1333] (ab108629)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1333] to TUBA1B
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-TUBA1B抗体[EPR1333]
参阅全部 TUBA1B 一抗
描述

兔单克隆抗体[EPR1333] to TUBA1B
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HeLa, HEK-293, Jurkat, 293T, A431, Mouse brain, Mouse kidney, and Rat brain lysates. IHC-P: human breast carcinoma, placenta and breast tissues, mouse liver, and rat liver tissues. ICC/IF: Jurkat and HeLa cells. Flow Cyt (intra): Jurkat cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR1333
同种型

IgG
研究领域

Alternative Versions
- Anti-TUBA1B antibody [EPR1333] - BSA and Azide free (ab247709)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
Recombinant Protein
- Recombinant Human TUBA1B protein (ab160967)

The Abpromise guarantee

Abpromise™承诺保证使用ab108629于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB		1/100000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). For unpurified use at 1/10000 - 1/50000.
IHC-P		1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000.
ICC/IF		1/50. For unpurified use at 1/250 - 1/500.

说明
Flow Cyt (Intra) 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/100000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). For unpurified use at 1/10000 - 1/50000.
IHC-P 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/500 - 1/1000.
ICC/IF 1/50. For unpurified use at 1/250 - 1/500.

应用说明

Is unsuitable for IP.

功能

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
序列相似性

Belongs to the tubulin family.
翻译后修饰

Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
细胞定位

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession P68363 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10376 Human
- Entrez Gene: 22143 Mouse
- Entrez Gene: 500929 Rat
- Omim: 602530 Human
- SwissProt: P68363 Human
- SwissProt: P05213 Mouse
- SwissProt: Q6P9V9 Rat
- Unigene: 524390 Human
- Unigene: 392113 Mouse
- Unigene: 99661 Rat
see all
别名
- Alpha tubulin ubiquitous antibody
- Alpha-tubulin ubiquitous antibody
- K alpha 1 antibody
- TBA1B_HUMAN antibody
- TUBA1B antibody
- Tubulin alpha 1B antibody
- Tubulin alpha 1B chain antibody
- Tubulin alpha ubiquitous antibody
- Tubulin alpha ubiquitous chain antibody
- Tubulin alpha-1B chain antibody
- Tubulin alpha-ubiquitous chain antibody
- Tubulin K alpha 1 antibody
- Tubulin K-alpha-1 antibody
see all

Western blot - Anti-TUBA1B antibody [EPR1333] (ab108629)

All lanes : Anti-TUBA1B antibody [EPR1333] (ab108629) at 1/100000 dilution (Purified)

Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TUBA1B antibody [EPR1333] (ab108629)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TUBA1B with purified ab108629 at 1/50 dilution (2.56 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TUBA1B with purified ab108629 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry (Intracellular) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TUBA1B with purified ab108629 at 1/20 dilution (5 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-TUBA1B antibody [EPR1333] (ab108629)

Anti-TUBA1B antibody [EPR1333] (ab108629) at 1/100000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TUBA1B with purified ab108629 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-TUBA1B antibody [EPR1333] (ab108629)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) labelling TUBA1B with purified ab108629 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TUBA1B with purified ab108629 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Unpurified ab108629, at 1/500 dilution, staining TUBA1B in paraffin embedded Human placenta tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUBA1B antibody [EPR1333] (ab108629)

Unpurified ab108629, at a 1/500 dilution, staining TUBA1B in paraffin embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TUBA1B antibody [EPR1333] (ab108629)

Unpurified ab108629, at a 1/250 dilution, staining TUBA1B in HeLa cells by Immunofluorescence.
Anti-TUBA1B antibody [EPR1333] (ab108629)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab108629？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab108629 被引用在 19 文献中.

Zhang K & Yuan E Combined analysis of bulk and single-cell RNA sequencing reveals novel natural killer cell-related prognostic biomarkers for predicting immunotherapeutic response in hepatocellular carcinoma. Front Immunol 14:1142126 (2023). PubMed: 37056756
Meng H et al. The expansion of newborn neurons in hippocampus improves social recognition deficit in a mouse model of autism. Front Psychiatry 14:1162179 (2023). PubMed: 37215664
Liu H et al. Validation of superior reference genes in mouse submandibular glands under developmental and functional regeneration states. Int J Mol Med 50:N/A (2022). PubMed: 36069228
Prentzell MT et al. G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling. Cell 184:655-674.e27 (2021). PubMed: 33497611
Hausrat TJ et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development. Dev Neurobiol 81:333-350 (2021). PubMed: 32293117

View all Publications for this product

客户评价及客户问答

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (19)

客户评价及客户问答