Anti-TTI1抗体- N-terminal (ab176696)
Key features and details
- Rabbit polyclonal to TTI1 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TTI1抗体- N-terminal
参阅全部 TTI1 一抗 -
描述
兔多克隆抗体to TTI1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide within Human TTI1 aa 1-50 (N terminal). The exact sequence is proprietary.
Sequence:MAVFDTPEEA FGVLRPVCVQ LTKTQTVENV EHLQTRLQAV SDSALQELQQ
Database link: O43156 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176696于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 122 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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相关性
TTI1 promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. TTI1 interacts with TELO2, MTOR, ATM, ATR, PRKDC, SMG1 and TRRAP. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 458235 Chimpanzee
- Entrez Gene: 534917 Cow
- Entrez Gene: 9675 Human
- Entrez Gene: 75425 Mouse
- Entrez Gene: 100154931 Pig
- Entrez Gene: 499935 Rat
- Omim: 614425 Human
- SwissProt: O43156 Human
see all -
别名
- TTI1 antibody
- KIAA0406 antibody
- smg 10 antibody
see all
图片
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Western blot - Anti-TTI1 antibody - N-terminal (ab176696)All lanes : Anti-TTI1 antibody - N-terminal (ab176696) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-TTI1 antibody - N-terminal (ab176696)Immunoprecipitation. ab176696 at 0.4 µg/ml staining TTI1 in HeLa whole cell lysate immunoprecipitated using ab176696 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
数据表及文件
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Datasheet download
文献 (2)
ab176696 被引用在 2 文献中.
- Zhang LX et al. TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway. Cancer Sci 114:855-869 (2023). PubMed: 36403197
- Wang R et al. Novel Compound Heterozygous Mutations in TTI2 Cause Syndromic Intellectual Disability in a Chinese Family. Front Genet 10:1060 (2019). PubMed: 31737043
