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AB181116

重组Anti-TTF2抗体[EPR12756]

Anti-TTF2 antibody [EPR12756]

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Rabbit Recombinant Monoclonal TTF2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

查看别名

Transcription termination factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, F2, HuF2, TTF2

5 Images
Western blot - Anti-TTF2 antibody [EPR12756] (AB181116)
  • WB

Supplier Data

Western blot - Anti-TTF2 antibody [EPR12756] (AB181116)

All lanes:

Western blot - Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilution

Lane 1:

Daudi cell line lysate at 20 µg

Lane 2:

HeLa cell line lysate at 20 µg

Lane 3:

Jurkat cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 130 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (AB181116)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (AB181116)

Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (AB181116)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (AB181116)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain : Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] (AB181116)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] (AB181116)

Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain : Dapi (blue)

Flow Cytometry (Intracellular) - Anti-TTF2 antibody [EPR12756] (AB181116)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TTF2 antibody [EPR12756] (AB181116)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed byGoat anti rabbit IgG (FITC) at 1/150 dilution (pink).Rabbit monoclonal IgG was used as Isotype control (green).

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12756

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TTF2 also known as transcription termination factor 2 is a protein involved in the removal of RNA polymerase II from chromatin during the process of transcription termination. The protein has a molecular mass of approximately 87 kDa. TTF2 shows expression in various tissues including high levels in the testis and lower levels in the brain and heart. Its activity is essential for ensuring that proper termination signals within the genomic DNA are recognized and that transcription is concluded accurately without inappropriate elongation.
Biological function summary

TTF2 plays a significant role in the dissociation of the transcription machinery ensuring effective recycling of RNA polymerase II for reuse in new transcription cycles. TTF2 is not a part of a multi-protein complex but works closely with other factors in post-transcriptional regulation. This protein facilitates the resetting of the chromatin environment which is important for the maintenance of gene expression fidelity across cellular processes.

Pathways

TTF2 interacts with several important transcriptional regulation pathways. It plays an integral role in the RNA polymerase II transcription termination pathway ensuring proper end of transcription. Furthermore its functional association with proteins like RNA polymerase II and termination-related cofactors emphasizes its role in efficient transcriptional control and regulatory processes that affect gene expression.

TTF2 has been implicated in various cancers where transcriptional misregulation is a contributing factor. Changes or anomalies in TTF2 expression or function can disrupt normal transcription termination leading to aberrant gene expression and possibly promoting oncogenesis. Additionally TTF2's interaction with RNA polymerase II highlights its potential involvement in neurodegenerative disorders where precise gene regulation is affected. Understanding TTF2's role in these contexts can benefit the development of targeted therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
See full target information TTF2
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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