重组Anti-TTF1/Nkx2-1抗体[EPR5955(2)] (ab133737)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5955(2)] to TTF1/Nkx2-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TTF1/Nkx2-1抗体[EPR5955(2)]
参阅全部 TTF1/Nkx2-1 一抗 -
描述
兔单克隆抗体[EPR5955(2)] to TTF1/Nkx2-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Human fetal lung, Rat lung and Mouse lung lysates; Human lung adenocarcinoma and Human papillary carcinoma of thyroid gland tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.50 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5955(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133737于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 38-42 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. -
组织特异性
Thyroid and lung. -
疾病相关
Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems. -
序列相似性
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7080 Human
- Entrez Gene: 21869 Mouse
- Entrez Gene: 25628 Rat
- Omim: 600635 Human
- SwissProt: P43699 Human
- SwissProt: P50220 Mouse
- SwissProt: P23441 Rat
- Unigene: 94367 Human
see all -
别名
- AV026640 antibody
- BCH antibody
- Benign chorea antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal lung lysate
Lane 3 : Rat lung lysate
Lane 4 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis of paraffin embedded Human papillary carcinoma of thyroid gland tissue labelling TTF1/Nkx2-1 with ab133737 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing negative staining in human Normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing negative staining in human Glioma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing negative staining in human Breast carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing positive staining in human Lung adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland carcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1/Nkx2-1 antibody [EPR5955(2)] (ab133737)
Immunohistochemical analysis using ab133737 showing positive staining in human Thyroid gland tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab133737 被引用在 20 文献中.
- Sarrafha L et al. Novel human pluripotent stem cell-derived hypothalamus organoids demonstrate cellular diversity. iScience 26:107525 (2023). PubMed: 37646018
- Kraft BD et al. Alveolar Mitochondrial Quality Control During Acute Respiratory Distress Syndrome. Lab Invest 103:100197 (2023). PubMed: 37307952
- Kong Y et al. The kinase PLK1 promotes the development of Kras/Tp53-mutant lung adenocarcinoma through transcriptional activation of the receptor RET. Sci Signal 15:eabj4009 (2022). PubMed: 36194647
- Ge Z et al. Compared with High-intensity Interval Exercise, Moderate Intensity Constant Load Exercise is more effective in curbing the Growth and Metastasis of Lung Cancer. J Cancer 13:1468-1479 (2022). PubMed: 35371324
- Hamad SH et al. TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice. Oncogene 41:3423-3432 (2022). PubMed: 35577980