Anti-TTC11/FIS1抗体(ab96764)
Key features and details
- Rabbit polyclonal to TTC11/FIS1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-TTC11/FIS1抗体
参阅全部 TTC11/FIS1 一抗 -
描述
兔多克隆抗体to TTC11/FIS1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant fragment corresponding to Human TTC11/FIS1 aa 1-122.
Database link: NP_057152 -
阳性对照
- Raji whole cell lysate; NCIN87 xenograft; 293T, HeLa and Molt-4 lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96764于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
|
| IHC-P |
1/100 - 1/500.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
|
IHC-P
1/100 - 1/500. |
靶标
-
功能
Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. -
序列相似性
Belongs to the FIS1 family.
Contains 1 TPR repeat. -
结构域
The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L. -
翻译后修饰
Ubiquitinated by MARCH5. -
细胞定位
Mitochondrion outer membrane. Peroxisome membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 615565 Cow
- Entrez Gene: 51024 Human
- Entrez Gene: 66437 Mouse
- Entrez Gene: 288584 Rat
- Omim: 609003 Human
- SwissProt: Q3T0I5 Cow
- SwissProt: Q9Y3D6 Human
- SwissProt: Q9CQ92 Mouse
see all -
别名
- 2010003O14Rik antibody
- CGI 135 antibody
- CGI135 antibody
see all
图片
-
Western blot - Anti-TTC11/FIS1 antibody (ab96764)Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 17 kDa
15% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (ab96764)Immunohistochemical analysis of TTC11/FIS1 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.
-
Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (ab96764)ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (21)
ab96764 被引用在 21 文献中.
- Shao SM et al. Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid β25-35-Induced Alzheimer's Disease Mouse Model. Evid Based Complement Alternat Med 2022:3849441 (2022). PubMed: 35463064
- Harmuth T et al. Mitochondrial Dysfunction in Spinocerebellar Ataxia Type 3 Is Linked to VDAC1 Deubiquitination. Int J Mol Sci 23:N/A (2022). PubMed: 35682609
- Chen W et al. Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect. Int J Nanomedicine 17:5431-5446 (2022). PubMed: 36426375
- Kitaoka Y et al. Voluntary exercise prevents abnormal muscle mitochondrial morphology in cancer cachexia mice. Physiol Rep 9:e15016 (2021). PubMed: 34427401
- Takahashi K et al. Lactate administration does not affect denervation-induced loss of mitochondrial content and muscle mass in mice. FEBS Open Bio 11:2836-2844 (2021). PubMed: 34510821