Anti-TSGA2抗体(ab224386)
Key features and details
- Rabbit polyclonal to TSGA2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TSGA2抗体
参阅全部 TSGA2 一抗 -
描述
兔多克隆抗体to TSGA2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human TSGA2 aa 200 to the C-terminus.
Database link: Q8WYR4 -
阳性对照
- IHC-P: Human fallopian tube and testis tissue. WB: TSGA2 overexpression HEK-293T lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224386于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.
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| 说明 |
|---|
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa. |
靶标
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相关性
TSGA2 (Testis specific gene A2), may play an important role in male meiosis. Its expression is restricted to the radial spokes of the axonemes of both sperm and cilia. -
细胞定位
Cytoplasmic. Note: Cytoplasmic in late spermatocytes, secondary spermatocytes and round spermatids. Gathered around metaphase chromosomes during meiotic divisions. -
数据库链接
- Entrez Gene: 89765 Human
- Omim: 609314 Human
- SwissProt: Q8WYR4 Human
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别名
- Cancer/testis antigen 79 antibody
- CT 79 antibody
- CT79 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (ab224386)
Paraffin-embedded human testis tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.
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Western blot - Anti-TSGA2 antibody (ab224386)All lanes : Anti-TSGA2 antibody (ab224386) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : TSGA2 overexpression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 35 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (ab224386)Paraffin-embedded human fallopian tube tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab224386 尚未被引用在任何文献中。