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重组RabMAb

重组Anti-TSG6抗体[EPR17582-45] - BSA and Azide free (ab274383)

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Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
  • Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
  • Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
  • Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
  • Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17582-45] to TSG6 - BSA and Azide free
  • Suitable for: WB, Flow Cyt (Intra), IP
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-TSG6抗体[EPR17582-45] - BSA and Azide free
    参阅全部 TSG6 一抗

  • 描述

    兔单克隆抗体[EPR17582-45] to TSG6 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, Flow Cyt (Intra), IPmore details
    不适用于: ICC/IF or IHC-P

  • 种属反应性

    与反应: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours,) whole cell lysate; Rat amniotic membrane tissue lysate. Flow Cyt (intra): THP-1 (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) cells. IP: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate.

  • 常规说明

    ab274383 is the carrier-free version of ab267469.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274383于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 36, 120 kDa (predicted molecular weight: 31 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 36, 120 kDa (predicted molecular weight: 31 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF or IHC-P.

靶标

  • 功能

    Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

  • 组织特异性

    Found in the synovial fluid of patients with rheumatoid arthritis.

  • 序列相似性

    Contains 1 CUB domain.
    Contains 1 Link domain.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Hyaluronate binding protein antibody
      • Hyaluronate-binding protein antibody
      • TNF alpha induced protein 6 antibody
      • TNF alpha-induced protein 6 antibody
      • TNF stimulated gene 6 protein antibody
      • TNF-stimulated gene 6 protein antibody
      • TNFAIP 6 antibody
      • Tnfaip6 antibody
      • TSG 6 antibody
      • TSG-6 antibody
      • TSG6_HUMAN antibody
      • Tumor necrosis factor alpha induced protein 6 antibody
      • Tumor necrosis factor alpha inducible protein 6 antibody
      • Tumor necrosis factor alpha-induced protein 6 antibody
      • Tumor necrosis factor inducible gene 6 protein antibody
      • Tumor necrosis factor stimulated gene 6 protein antibody
      • Tumor necrosis factor-inducible gene 6 protein antibody
      see all

    图片

    • Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution + Rat amniotic membrane tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 31 kDa
      Observed band size: 120,36 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

      The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469)

    • Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) (Red) / Untreated control (Green) cells labelling TSG6 with ab267469 at 1/500 dilution (0.1ug) (Red, Green) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469). 

       

       

    • Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

      TSG6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate with ab267469 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267469 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate 10 ug.

      Lane 2: ab267469 IP in THP-1 (treated as above).

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267469 in THP-1 (treated as above) whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 41 seconds

      This blot was developed using a higher sensitivity ECL substrate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469).

    • Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      All lanes : Anti-TSG6 antibody [EPR17582-45] (ab267469) at 1/1000 dilution

      Lane 1 : THP-1 (human monocytic leukemia monocyte), whole cell lysate
      Lane 2 : THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours), whole cell lysate
      Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
      Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 120,36 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).

      The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).

      Negative control: A549 (PMID: 19389798).

      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267469).

    • Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
      Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab274383？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab274383 尚未被引用在任何文献中。

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