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Anti-Tryptophan Hydroxylase/TPH (phospho S260)抗体(ab30574)

Submit a review Q&A (3)References (4)
Western blot - Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)

    Key features and details

    • Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260)
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Tryptophan Hydroxylase/TPH (phospho S260)抗体
      参阅全部 Tryptophan Hydroxylase/TPH 一抗

    • 描述

      兔多克隆抗体to Tryptophan Hydroxylase/TPH (phospho S260)

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Rat

    • 免疫原

      Synthetic peptide corresponding to Rat Tryptophan Hydroxylase/TPH (phospho S260).
      Database link: P09810

    • 阳性对照

      • Rat brain stem lysate, human dorsal Raphe nucleus.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab30574于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
    说明
    WB
    1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

    靶标

    • 组织特异性

      Isoform 2 seems to be less widely expressed than isoform 1.

    • 通路

      Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.

    • 序列相似性

      Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Contains 1 ACT domain.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Indoleacetic acid 5 hydroxylase antibody
        • L tryptophan hydroxylase antibody
        • MGC119994 antibody
        • TPH 1 antibody
        • TPH antibody
        • TPH1 antibody
        • TPH1_HUMAN antibody
        • TPRH antibody
        • TRPH antibody
        • Tryptophan 5 hydroxylase 1 antibody
        • Tryptophan 5 monooxygenase 1 antibody
        • Tryptophan 5 monooxygenase antibody
        • Tryptophan 5-hydroxylase 1 antibody
        • Tryptophan 5-monooxygenase 1 antibody
        • Tryptophan hydroxylase 1 antibody
        see all

      图片

      • Western blot - Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)
        Western blot - Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574)
        All lanes : Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574) at 1/1000 dilution

        Lane 1 : rat brain stem lysate (control)
        Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
        Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.

        Lysates/proteins at 15 µg per lane.

        Predicted band size: 51 kDa
        Observed band size: 55 kDa why is the actual band size different from the predicted?
        Additional bands at: <43 kDa, 48 kDa, 59 kDa. We are unsure as to the identity of these extra bands.



        In lane 2, the labeling is specifically blocked by the S260 phosphopeptide used as antigen.
        In lane 3, the corresponding nonphosphopeptide did not block the immunolabeling.

      文献 (4)

      发表研究结果有使用 ab30574？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab30574 被引用在 4 文献中.

      • Herrera-Martínez AD  et al. Effect of the Tryptophan Hydroxylase Inhibitor Telotristat on Growth and Serotonin Secretion in 2D and 3D Cultured Pancreatic Neuroendocrine Tumor Cells. Neuroendocrinology 110:351-363 (2020). PubMed: 31319410
      • Damen R  et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One 8:e62607 (2013). PubMed: 23638125
      • Chin A  et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol 302:G397-405 (2012). WB ; Human . PubMed: 22038827
      • Haugen M  et al. Differential signal pathway activation and 5-HT function: the role of gut enterochromaffin cells as oxygen sensors. Am J Physiol Gastrointest Liver Physiol 303:G1164-73 (2012). WB ; Human . PubMed: 22936271

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      1-3 of 3 Abreviews or Q&A

      Question

      According to the datasheet for the product, Western blots using the Tryptophan Hydroxylase (phospho S260) antibody (ab30574) were done using Rat brain stem lysate which means that the antibody should recognize TPH2 as well as TPH1 as TPH2 is the neuronal form and TPH1 is located in the periphery and pineal. Do you know if this antobody recognizes TPH1 in the pineal?

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      Answer

      The sequence of the antigen for this antibody is conserved only in TPH1, however it may still recognize TPH2 although there is not 100 % homology with the TPH2 sequence. The ability of this antibody to recognize TPH2 specifically has not yet been directly tested. This antibody has not been directly tested in pineal extracts, and I am unsure of the stoichiometry of phosphorylated TPH in that region, but if there is sufficient phosphorylation of the s 260 in the pineal gland, then it should work. I hope this clarifies things. Please contact us again if you have any additional questions.

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      Question

      Does the western blot for this product reflect phosphorylation of untreated rat brain stem lysate or was the tissue treated with phosphorylating conditions to induce phosphorylation of the tryptophan hydroxylase enzyme?

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      Answer

      Thank you for your enquiry. I have been in touch with the source of this antibody and I can confirm that the lysate used for that blot was made from untreated rat brain. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Does this antibody have a specificity for either isoform TPH1 or TPH2?

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      Answer

      This antibody is specific for TPH1. Please contact us again if you have any additional questions.

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