重组Anti-Tryptophan Hydroxylase/TPH抗体[EP1311Y] (ab52954)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)](https://www.abcam.cn/ps/products/52/ab52954/Images/ab52954-8-anti-tryptophan-hydroxylaseep-antibody-ep1311y-immunohistochemistry-bladder-carcinoma-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase/TPH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tryptophan Hydroxylase/TPH抗体[EP1311Y]
参阅全部 Tryptophan Hydroxylase/TPH 一抗 -
描述
兔单克隆抗体[EP1311Y] to Tryptophan Hydroxylase/TPH -
宿主
Rabbit -
特异性
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human small intestine and pituitary tissue lysate. Mouse brain tissue lysate. Recombinant full length mouse Tph1 protein with C-terminal MYC/DDK tag. N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1311Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] - BSA and Azide free (ab227845)
- HRP Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab237450)
- APC Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319197)
- PE Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319345)
- Alexa Fluor® 488 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319479)
- Alexa Fluor® 647 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319592)
- Alexa Fluor® 594 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319696)
- Alexa Fluor® 555 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab319838)
- Alexa Fluor® 750 Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab321676)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52954于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
|
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| IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. For the unpurified format use at 1/50 dilution |
靶标
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组织特异性
Isoform 2 seems to be less widely expressed than isoform 1. -
通路
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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数据库链接
- Entrez Gene: 7166 Human
- Entrez Gene: 21990 Mouse
- Omim: 191060 Human
- SwissProt: P17752 Human
- SwissProt: P17532 Mouse
- Unigene: 591999 Human
- Unigene: 248684 Mouse
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别名
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human bladder carcinoma tissue sections labeling Tryptophan Hydroxylase/TPH with Purified ab52954 at 1:2000 dilution (1.191 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)](https://www.abcam.cn/ps/products/52/ab52954/Images/ab52954-472786-AntiTryptophanHydroxylaseTPHantibodyEP1311Ywesternblotrecombinantfragmenthuman.jpg)
Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)Lane 1 : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 2 : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab205606) at 1/1000 dilution
All lanes : Full-length Tph1 mouse recombinant protein with C-terminal MYC/DDK tag 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)](https://www.abcam.cn/ps/products/52/ab52954/Images/ab52954-472785-AntiTryptophanHydroxylaseTPHantibodyEP1311Ywesternblotintestinepituitaryhumanbrainmouse.jpg)
Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)All lanes : Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution
Lane 1 : Human small intestine tissue lysate
Lane 2 : Human pituitary tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 180 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
TPH1 is mainly expressed in non-neuronal peripheral tissues like pineal gland.
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![Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)](https://www.abcam.cn/ps/products/52/ab52954/Images/ab52954-477454-antitryptophan-hydroxylase-tph-recombinant-protein-human.jpg)
Western blot - Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954) at 1/1000 dilution + N-His tagged human Tryptophan Hydroxylase/TPH (aa 105 to 420) recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)](https://www.abcam.cn/ps/products/52/ab52954/Images/ab52954-4-benefits-of-recombinant-antibodies.png)
Anti-Tryptophan Hydroxylase/TPH antibody [EP1311Y] (ab52954)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab52954 被引用在 25 文献中.
- Omoluabi T et al. Novelty-like activation of locus coeruleus protects against deleterious human pretangle tau effects while stress-inducing activation worsens its effects. Alzheimers Dement (N Y) 7:e12231 (2021). PubMed: 35005208
- Tarbit E et al. Increased release of serotonin from rat primary isolated adult cardiac myofibroblasts. Sci Rep 11:20376 (2021). PubMed: 34645867
- Yi G et al. A study of the mechanism of small-molecule soybean-protein-derived peptide supplement to promote sleep in a mouse model. RSC Adv 10:11264-11273 (2020). PubMed: 35495343
- Chen DQ et al. Poricoic acid A as a modulator of TPH-1 expression inhibits renal fibrosis via modulating protein stability of ß-catenin and ß-catenin-mediated transcription. Ther Adv Chronic Dis 11:2040622320962648 (2020). PubMed: 33062239
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309