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AB17263

Anti-Trypsin 抗体 [2C4]

Anti-Trypsin antibody [2C4]

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(3 Publications)

Mouse Monoclonal Trypsin antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human PRSS1.

查看别名

TRP1, TRY1, TRYP1, PRSS1, Serine protease 1, Anionic trypsin I, Anionic trypsin-I, Beta-trypsin, Cationic trypsinogen, Pretrypsinogen I, Trypsin I, Trypsin-1

1 Images
Western blot - Anti-Trypsin antibody [2C4] (AB17263)
  • WB

Unknown

Western blot - Anti-Trypsin antibody [2C4] (AB17263)

All lanes:

Western blot - Anti-Trypsin antibody [2C4] (ab17263) at 5 µg/mL

Lane 1:

Western blot - Native human Trypsin protein (<a href='/products/proteins-peptides/native-human-trypsin-protein-ab78527'>ab78527</a>) at 1 µg

Lane 2:

Western blot - Native human Trypsin protein (<a href='/products/proteins-peptides/native-human-trypsin-protein-ab78527'>ab78527</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 27 kDa

true

Exposure time: 20min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2C4

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Native Full Length Protein corresponding to Human PRSS1.

P07477

特异性

This antibody is specific for human trypsin, trypsinogen and trypsin in complex with alpha 1 antitrypsin.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.
Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
See full target information PRSS1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:2714-2731 PubMed37424812

2023

Long non-coding RNA enhances gastric carcinoma proliferation, migration and invasion via the miR-136-5p/HOXC10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingzhang Li,Kai Sun,Mingming Zhang,Yusheng Luo,Ying Zhang,Cheng Huang,Juanjuan Hong,Dongmei Chen,Wangjun Liao

The Analyst 145:5801-5807 PubMed32692324

2020

Picoliter enzyme reactor on a nanofluidic device exceeding the bulk reaction rate.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Yamamoto,Kyojiro Morikawa,Hiroyuki Imanaka,Koreyoshi Imamura,Takehiko Kitamori

Pharmaceutical research 29:332-41 PubMed21818710

2011

Investigations into the fate of inhaled salmon calcitonin at the respiratory epithelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Baginski,Frederic Tewes,Stephen T Buckley,Anne Marie Healy,Udo Bakowsky,Carsten Ehrhardt
View all publications
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替代产品

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AB211491

Anti-Trypsin antibody [EPR19498-15]

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Primary Antibodies

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搭配使用产品

Assay Kits

AB102531

Trypsin Activity Assay Kit (Colorimetric)

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

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Secondary Antibodies

AB150077

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Western blot - Anti-MMP9 antibody [EP1254] (AB76003)

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Primary Antibodies

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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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