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AB305164

重组Anti-Trypsin 1抗体[EPR24510-117] - BSA and Azide free (Capture)

Anti-Trypsin 1 antibody [EPR24510-117] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Trypsin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

TRP1, TRY1, TRYP1, PRSS1, Serine protease 1, Anionic trypsin I, Anionic trypsin-I, Beta-trypsin, Cationic trypsinogen, Pretrypsinogen I, Trypsin I, Trypsin-1

1 Images
Sandwich ELISA - Anti-Trypsin 1 antibody [EPR24510-117] - BSA and Azide free (Capture) (AB305164)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Trypsin 1 antibody [EPR24510-117] - BSA and Azide free (Capture) (AB305164)

Sandwich ELISA of ab305096 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24510-117

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.
Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
See full target information PRSS1
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搭配使用产品

Assay Kits

AB102531

Trypsin Activity Assay Kit (Colorimetric)

Functional Studies - Trypsin Activity Assay Kit (Colorimetric) (AB102531)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB205921

Anti-PD-L1 antibody [28-8]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PD-L1 antibody [28-8] (AB205921)

  • 4
  • 15
Primary Antibodies

AB76003

Anti-MMP9 antibody [EP1254]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MMP9 antibody [EP1254] (AB76003)

  • 4
  • 12
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

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