Anti-TRPV1抗体(ab6166)
Key features and details
- Rabbit polyclonal to TRPV1
- Suitable for: WB, IHC-FrFl
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-TRPV1抗体
参阅全部 TRPV1 一抗 -
描述
兔多克隆抗体to TRPV1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-FrFlmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Rat TRPV1 aa 800-900 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
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常规说明
This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 94.976 kDa.
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| IHC-FrFl |
1/100 - 1/2000.
Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining. |
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 94.976 kDa. |
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IHC-FrFl
1/100 - 1/2000. Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining. |
靶标
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功能
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
组织特异性
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
结构域
The association domain (AD) is necessary for self-association. -
翻译后修饰
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 193034 Mouse
- SwissProt: Q704Y3 Mouse
- Unigene: 447485 Mouse
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别名
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
图片
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Western blot - Anti-TRPV1 antibody (ab6166)Anti-TRPV1 antibody (ab6166) at 1/1000 dilution + Mouse dorsal root ganglia at 20 µg
Predicted band size: 94.976 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.
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Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.
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Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution.
实验方案
数据表及文件
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Datasheet download
文献 (24)
ab6166 被引用在 24 文献中.
- Oh SJ et al. TRPV1 inhibition overcomes cisplatin resistance by blocking autophagy-mediated hyperactivation of EGFR signaling pathway. Nat Commun 14:2691 (2023). PubMed: 37165076
- Zhang J et al. Analgesic and potentiated photothermal therapy with ropivacaine-loaded hydrogels. Theranostics 13:2226-2240 (2023). PubMed: 37153743
- He J et al. Serum/glucocorticoid-regulated kinase 1-targeted transient receptor potential oxalate subtype 1 regulates bladder smooth muscle cell proliferation due to bladder outlet obstruction in mice via activated T cell nuclear factor transcription factor 2. IUBMB Life 74:463-473 (2022). PubMed: 35148462
- Wang X et al. Study on the intervention effect and mechanism of bacillus Calmette-Guerin polysaccharide and nucleic acid injection on atopic dermatitis by targeting the transient receptor potential vanilloid subtype 1 pathway. Ann Transl Med 10:608 (2022). PubMed: 35722408
- Wang X et al. TRPV1 Modulator Ameliorates Alzheimer-Like Amyloid-β Neuropathology via Akt/Gsk3β-Mediated Nrf2 Activation in the Neuro-2a/APP Cell Model. Oxid Med Cell Longev 2022:1544244 (2022). PubMed: 36065437
