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Anti-TRPV1抗体(ab3487)

Reviews (2)Q&A (2)References (23)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (ab3487)
  • Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab3487)

Key features and details

  • Rabbit polyclonal to TRPV1
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-TRPV1抗体
    参阅全部 TRPV1 一抗

  • 描述

    兔多克隆抗体to TRPV1

  • 宿主

    Rabbit

  • 特异性

    Immunohistochemical staining of TRPV1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of TRPV1 in human prostatic urethra results in fibrous staining within the epithelial cells.

  • 经测试应用

    适用于: IHC-Fr, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human TRPV1 aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-F: human skin tissue IHC-P: human glabrous skin tissue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3487于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (1)
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.
说明
IHC-Fr
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.

  • 组织特异性

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

  • 序列相似性

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.

  • 结构域

    The association domain (AD) is necessary for self-association.

  • 翻译后修饰

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

  • 细胞定位

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Capsaicin receptor antibody
      • DKFZp434K0220 antibody
      • osm 9 like TRP channel 1 antibody
      • Osm-9-like TRP channel 1 antibody
      • OTRPC1 antibody
      • Transient receptor potential cation channel subfamily V member 1 antibody
      • TRPV 1 antibody
      • Trpv1 antibody
      • TRPV1_HUMAN antibody
      • Vanilloid receptor 1 antibody
      • Vanilloid receptor subtype 1 antibody
      • VR 1 antibody
      • VR1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (ab3487)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPV1 antibody (ab3487)

      Immunolocalization of TRPV1 in human glabrous skin using ab3487.

    • Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab3487)
      Immunohistochemistry (Frozen sections) - Anti-TRPV1 antibody (ab3487)

      Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling TRPV1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

    文献 (23)

    发表研究结果有使用 ab3487？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab3487 被引用在 23 文献中.

    • Kiss F  et al. Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35163843
    • Cheng L  et al. Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease. Gastroenterol Res Pract 2022:7294775 (2022). PubMed: 35677724
    • Le TT  et al. Sensory nerves enhance triple-negative breast cancer invasion and metastasis via the axon guidance molecule PlexinB3. NPJ Breast Cancer 8:116 (2022). PubMed: 36333352
    • Guo Y  et al. Nutrient-induced hyperosmosis evokes vasorelaxation via TRPV1 channel-mediated, endothelium-dependent, hyperpolarisation in healthy and colitis mice. Br J Pharmacol 178:689-708 (2021). PubMed: 33169358
    • Chakrabarti S  et al. Functional Characterization of Ovine Dorsal Root Ganglion Neurons Reveal Peripheral Sensitization after Osteochondral Defect. eNeuro 8:N/A (2021). PubMed: 34544757
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-TRPV1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin, 3D skin model)
    Permeabilization
    Yes - 0.4% Triton X100
    Specification
    skin, 3D skin model
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 21 2011

    Immunohistochemistry free floating abreview for Anti-TRPV1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 10 2011

    Question

    I tried to test the indicate protocol : https://www.abcam.com/VR1-antibody-ab3487.html&tab=abreviews&intabreviewid=26192 : test fixation methanol, over night, goat serum saturation, permeabilization triton X100

    But without success !

    To answer to your question (Can you confirm that the other reactives (e.g. the secondary antibody) do work fine when used with other primary rabbit antibodies?) , yes, we use this secondary antibody frequently and it work very well.

    Please can you give me a solution, my client want a VR1 labelling on human skin !

    Thanks a lot

    Regards.

    Read More
    Answer

    Thank your email. I am very sorry that the suggestions did not helpto improve the results. I must therefore conclude, that the vial you received must have been damaged - maybe during storage or transport. I would be pleased to replace this vial for you, by either a credit/refund or a replacement vial. Please let me know which optionyou prefer andwhether you would like to accept either a new vial of ab3487 or another alternative antibody from our catalog?
    I apologize for the inconvenience. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Inquiry: Mr or Mrs I tried the antibody ab3487 (anti-VR1) on frozen section of skin : - fixed in aceton/methanol - saturation in milk - antibody dilution : 1/1000 and 1/100, 1 hour at RT - secondary antibody GAR alexa fluor 488, 1 hour at RT - wash in PBS-tween 20. It didn't work. Do you have a specific protocol for this marker ? Could you give me the conditions please ? Thanks a lot. Regards

    Read More
    Answer

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

    We do not have a specific protocol for this antibody. We expect this antibody to work with a general IHC protocol after the classic optimisation steps. Looking at the information you provided, I would like to offerthe followingsuggestions to help optimize the results from ab3487. I would also appreciate if you can confirm some further details:

    1.) Can you please confirm of which species the skin sections usedare coming from. We have tested this antibody so far only on human tissue. It might reacts with other species, however we need to test this first.

    2.) I can recommend to incubate the antibody overnight at 4°C. This can increase the staining intensity.

    3.) I can also recommend to test different blocking solutions. This can significantly improve the staining. I can recommend to test BSA and serum instead of milk. As serum you would use serum of the species of the secondary antibody (goat?). The researcher having submitted the feedback on this antibody, has used for example 10% serum. See his Abreview: https://www.abcam.com/VR1-antibody-ab3487.html&tab=abreviews&intabreviewid=26192

    4.) Can you confirm that the other reactives (e.g. the secondary antibody) do work fine when used with other primary rabbit antibodies?

    5.) Have you tested other fixation methods as well, such as Methanol only or Paraformaldehyde? The user mentionned above, had used succesfully Methanol only, whereas the lab has tested the antibody also in paraformaldhyde fixation obtaining good results.

    I hope these suggestions will help to identify the problem. Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

    Read More

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