Anti-TRPV1抗体(ab3487)
Key features and details
- Rabbit polyclonal to TRPV1
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRPV1抗体
参阅全部 TRPV1 一抗 -
描述
兔多克隆抗体to TRPV1 -
宿主
Rabbit -
特异性
Immunohistochemical staining of TRPV1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of TRPV1 in human prostatic urethra results in fibrous staining within the epithelial cells.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TRPV1 aa 1-100.
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阳性对照
- IHC-F: human skin tissue IHC-P: human glabrous skin tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-Fr
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis. -
组织特异性
Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level). -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats. -
结构域
The association domain (AD) is necessary for self-association. -
翻译后修饰
Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. -
细胞定位
Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7442 Human
- Omim: 602076 Human
- SwissProt: Q8NER1 Human
- Unigene: 579217 Human
- Unigene: 655380 Human
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别名
- Capsaicin receptor antibody
- DKFZp434K0220 antibody
- osm 9 like TRP channel 1 antibody
see all
图片
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Immunolocalization of TRPV1 in human glabrous skin using ab3487.
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Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling TRPV1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab3487 被引用在 23 文献中.
- Kiss F et al. Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35163843
- Cheng L et al. Expression of TRP Channels in Colonic Mucosa of IBS-D Patients and Its Correlation with the Severity of the Disease. Gastroenterol Res Pract 2022:7294775 (2022). PubMed: 35677724
- Le TT et al. Sensory nerves enhance triple-negative breast cancer invasion and metastasis via the axon guidance molecule PlexinB3. NPJ Breast Cancer 8:116 (2022). PubMed: 36333352
- Guo Y et al. Nutrient-induced hyperosmosis evokes vasorelaxation via TRPV1 channel-mediated, endothelium-dependent, hyperpolarisation in healthy and colitis mice. Br J Pharmacol 178:689-708 (2021). PubMed: 33169358
- Chakrabarti S et al. Functional Characterization of Ovine Dorsal Root Ganglion Neurons Reveal Peripheral Sensitization after Osteochondral Defect. eNeuro 8:N/A (2021). PubMed: 34544757