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Signal Transduction Metabolism Plasma Membrane Channels

Anti-TRPM8抗体(ab3243)

  • Datasheet
  • SDS
Reviews (5)Q&A (15)References (27)

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Western blot - Anti-TRPM8 antibody (ab3243)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (ab3243)
  • Western blot - Anti-TRPM8 antibody (ab3243)

Key features and details

  • Rabbit polyclonal to TRPM8
  • Suitable for: WB, IHC-P
  • Reacts with: Human, African green monkey
  • Isotype: IgG

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概述

  • 产品名称

    Anti-TRPM8抗体
    参阅全部 TRPM8 一抗
  • 描述

    兔多克隆抗体to TRPM8
  • 宿主

    Rabbit
  • 特异性

    Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein.
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human, African green monkey
  • 免疫原

    Synthetic peptide corresponding to Human TRPM8.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    Immunogen affinity purified.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3243于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.
IHC-P
1/10 - 1/2000.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.
IHC-P
1/10 - 1/2000.

靶标

  • 功能

    Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • 组织特异性

    Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • 序列相似性

    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession Q7Z2W7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 79054 Human
    • Omim: 606678 Human
    • SwissProt: Q7Z2W7 Human
    • Unigene: 366053 Human
    • 别名

      • Long transient receptor potential channel 6 antibody
      • LTrpC-6 antibody
      • LTrpC6 antibody
      • MGC2849 antibody
      • Short form of the TRPM8 cationic channel antibody
      • Transient receptor potential cation channel subfamily M member 8 antibody
      • Transient receptor potential p8 antibody
      • transient receptor potential-p8 antibody
      • Trp p8 antibody
      • Trp-p8 antibody
      • Trpm8 antibody
      • TRPM8_HUMAN antibody
      • TRPP8 antibody
      see all

    图片

    • Western blot - Anti-TRPM8 antibody (ab3243)
      Western blot - Anti-TRPM8 antibody (ab3243)
      Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate

      Predicted band size: 127 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (ab3243)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPM8 antibody (ab3243)

      ab3243  staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

    • Western blot - Anti-TRPM8 antibody (ab3243)
      Western blot - Anti-TRPM8 antibody (ab3243)Image courtesy of an anonymous AbReview
      All lanes : Anti-TRPM8 antibody (ab3243) at 1000 cells

      Lane 1 : Human liver tissue lysate
      Lane 2 : Human hepatocyte lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit HRP at 1/1000 dilution

      Predicted band size: 127 kDa

      See Abreview

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (27)

    发表研究结果有使用 ab3243?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3243 被引用在 27 文献中.

    • Xu Q  et al. Expression of transient receptor potential cation channel subfamily M member 8 in gastric cancer and its clinical significance. Exp Ther Med 21:377 (2021). PubMed: 33680099
    • Gao Y  et al. Saponins from Panax japonicus ameliorate age-related renal fibrosis by inhibition of inflammation mediated by NF-?B and TGF-ß1/Smad signaling and suppression of oxidative stress via activation of Nrf2-ARE signaling. J Ginseng Res 45:408-419 (2021). PubMed: 34025134
    • Wen J  et al. Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice. J Exp Biol 223:N/A (2020). PubMed: 32341176
    • Silva DF  et al. TRPM8 channel activation triggers relaxation of pudendal artery with increased sensitivity in the hypertensive rats. Pharmacol Res 147:104329 (2019). PubMed: 31340190
    • Liu T  et al. RNA interference-mediated depletion of TRPM8 enhances the efficacy of epirubicin chemotherapy in prostate cancer LNCaP and PC3 cells. Oncol Lett 15:4129-4136 (2018). PubMed: 29541177
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 20 Abreviews or Q&A

    Flow Cytometry abreview for Anti-TRPM8 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (DU145)
    Permeabilization
    Yes - Leukoperm B (Bio-Rad)
    Gating Strategy
    Live cells using FSC/SSC
    Specification
    DU145
    Preparation
    Cell harvesting/tissue preparation method: Cells were harvested using TrypLE express enzyme and washed into FACS buffer (Ca2+ and Mg2+ -free PBS containing 2% FBS, 0.02% NaN3 and 1mM EDTA). Cells were fixed and then incubated in permeabilisation buffer containing Human TruStain FcX (Biolegend) before addition of the primary antibody.
    Sample buffer: Ca2+ and Mg2+ -free PBS containing 2% FBS, 0.02% NaN3 and 1mM EDTA
    Fixation
    Leukoperm A (Bio-Rad)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 04 2020

    Western blot abreview for Anti-TRPM8 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    human hepatocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 29 2017

    Immunoprecipitation abreview for Anti-TRPM8 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A
    Sample
    Human Cell lysate - whole cell (Hek cells transfected with human TRPM8)
    Specification
    Hek cells transfected with human TRPM8
    Total protein in input
    800 µg
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Larisa Maier

    Verified customer

    提交于 Sep 17 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-TRPM8 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin, HR-1)
    Specification
    skin, HR-1
    Fixative
    Methanol
    Permeabilization
    Yes - 0.4% TritonX100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 20 2011

    Immunohistochemistry (Frozen sections) abreview for Anti-TRPM8 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (prostate cancer)
    Specification
    prostate cancer
    Blocking step
    Other as blocking agent for 30 minute(s) · Concentration: 1.2%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Gabriel Bidaux

    Verified customer

    提交于 Aug 21 2006

    Question

    Estimada ,
    Tras recibir su respuesta a mi pregunta, quisiera volver a pedirle si por favor podría mandarme la información del anticuerpo AB3243 que antes comercializaban. Se trata de un anticuerpo del que aun disponemos en el laboratorio y seguimos usando, por lo que agradecería me enviaran la información detallada del mismo.
    De los anticuerpos recomendados, no se especifica que sea específico para TRPM8 humano, podría confirmármelo?
    Muchas gracias

    Read More

    Abcam community

    Verified customer

    Asked on Jun 11 2012

    Answer

    Gracias por contactarnos, le pido disculpas por la espera.

    Adjunto al email la datasheet del producto descatalogado ab3243. Al no estar disponible no tengo acceso a la datasheet en formato pdf, por lo que la envío copiada en un documento Word, siento los inconvenientes.

    También le envío el enlace a la búsqueda llevada a cabo de anticuerpos anti TRPM8 humano en WB y IHC (Fr/P).

    https://www.abcam.com/index.html?pageconfig=searchresults&search=TRPM8&pt=1&sk=app&sv=74&sn=IHC-P&l=4&fViewMore=1

    Si necesitara más información acerca de cualquiera de los productos identificados, un presupuesto o tuviera cualquier otra consulta, por favor, no dude en volvernos a contactar.

    Read More

    Abcam Scientific Support

    回复于 Jun 11 2012

    Question

    What was the protocol used to test ab3243 in IHC?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2011

    Answer

    Thank you for contacting Abcam. Please find the protocol used to test ab3243 in IHC below. If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Oct 26 2011

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM For the TRPM8 a size of about 130 kDa is predicted for human and also rat protein. However Xiao-Ru Yang et al found a band at 250 kDa as TRPM8 (see attachment). What do you think about these data and which size do you predict? I already tried tongue and bladder of rat as positive control. Unfortunately I find several unspecific bands. Does abcam provide an adequate positive control in order to find out it the signal comes at 250 or 130 kDa? Thank you very much for your help. SAMPLE Rat, adrenal medulla, ductus deferens PRIMARY ANTIBODY Abcam Anti TRPM8 POSITIVE AND NEGATIVE CONTROLS USED Positive control: tongue, bladder of rat ANTIBODY STORAGE CONDITIONS -20°C dissolved in BSA/TBST/NaN3 --> 4°C SAMPLE PREPARATION Preparation with TRIS/SDS, heating at 95°C, 5 min. Then loading buffer plus protein again heated at 95°C for 5 min. AMOUNT OF PROTEIN LOADED 70 µg ELECTROPHORESIS/GEL CONDITIONS 30:0,8 Acrylamide 8% TRANSFER AND BLOCKING CONDITIONS Blocking agent:5% milk/TBST (BSA not yet tryed) overnight SECONDARY ANTIBODY non Abcam, Peroxidase-conjugated Goat AntiRabbit

    Read More

    Abcam community

    Verified customer

    Asked on Aug 07 2007

    Answer

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab3243 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. I can confirm that our laboratory tested this antibody with transfected Cos cell lysate in western blotting and managed to obtain a band at around 130kDa. Unfortunately, I am unable to retrieve any further protocol information (amount of protein loaded, etc.) and therefore, the image is not suitable for use on our datasheet. I have attached a copy of the image for your reference only. I am unsure of the exact protocols used by Yang et al 2006, but please rest assured that we guarantee our products to perform well in species and applications as stated on the datasheet. If you would like some technical support, I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. In most cases, the cause of multiple bands is because the primary and/or secondary antibody concentration is too high and causes non-specific binding. What was the dilution that you used? The dilution that we have on the datasheet is a recommended starting dilution and customers are encouraged to determine the optimal concentration/dilution. You can try decreasing the primary (1:2000 ~ 1:5000) and secondary antibody concentration or run a no-primary control (without the primary antibody). Running a no-primary control will be able to eliminate the possibility that your secondary antibody is binding non-specifically. Another possible cause of non-specific bands is that the amount of protein is too much. We normally recommend using 20-40 micrograms per well. Please try this amount if you have not already done so. What are the band sizes you obtained? The bands located above the expected molecular weight band could be multimers. Please try boiling your protein in SDS-PAGE for 10 minutes rather than 5 minutes to ensure proper disruption of multimers. Meanwhile, bands located below the expected molecular weight may indicate that your target protein has been digested. Please make sure that you incorporated sufficient protease inhibitors and have kept your samples on ice the whole time. Increasing the washing frequency and duration would also help eliminate non-specific bands. Can you confirm that you have used Tween in your washing solution and have washed at least 15min x 3 times? I hope the above recommendations may already help you, if you still experience problems please do not hesitate to contact me with the answers to the above questions and an image of the blot if possible. I would also appreciate if you could also provide details of your order (purchase order number, shipping address/purchasing agent info, contact information, etc.) so that I can immediately arrange for a replacement or refund to you. Also, please advice on how you would to proceed with this enquiry. I look forward to hearing from you again in order to resolve the matter.

    Read More

    Abcam Scientific Support

    回复于 Aug 07 2007

    Question

    I followed your suggestions to perform western blot by using 3% BSA for blocking overnight and washing with TBST (0.1% Tween 20). Please find the Attached file that shows the result of western blot. The adjustment of the experiment condictions did not improve my results much.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2007

    Answer

    Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you purchased the antibody in the last 3 months. Can you please confirm your order details and what you would prefer? I look forward to hearing from you to arrange this,

    Read More

    Abcam Scientific Support

    回复于 Feb 08 2007

    Question

    Enclosed please find the western blot result by using ab3243. The exprimental condictions are as follows: 1. Loading total protein 40ug/lane in 9% separation SDS-PAGE gel (The second lane: bladder, as my postive control). 2. transfer to PVDF membrane (Millpore immobilon-FL), 47 Volt for 60 min. 3. blocking: 5% skim milk in TBST (0.05% Tween 20) 4. primary antibody: ab3243 (lot: 237021) by using 0.1ug/ml as final concentration at 4oC overnight. 5. secondary antibody: goat anti-rabbit HRP (Pierce) 1:2500 dilution at room temperature for 1 hour. 6. ECL: Supersignal west Pico Chemiluminescent (Pierce 34080)

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2007

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback, but not western blotting. You are obtaining an alarming number of extraneous bands. I would like to recommend that in order to obtain more specific results that you use 3% BSA as a blocking agent. This is routinely employed by our lab team. I would also like to recommend that you increase the percentage of Tween 20 to 0.1% in an effort to address the non-specific nature of this antibody. I would also like to recommend that you perform an overnight block of your membrane using this agent. If this does not improve your results please get back in touch with me and I am happy to provide you with a replacement antibody or credit against your purchase provided that you purchased this antibody within the past 90 days.

    Read More

    Abcam Scientific Support

    回复于 Jan 31 2007

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