重组Anti-TRPM7 + TRPM6抗体[EPR4582] (ab109438)
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.cn/ps/products/109/ab109438/Images/ab109438-122283-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg "Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4582] to TRPM7 + TRPM6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRPM7 + TRPM6抗体[EPR4582] -
描述
兔单克隆抗体[EPR4582] to TRPM7 + TRPM6 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 86% homology with TRPM6 (ten amino acid stretch with 100% homology). ab109438 has been shown to cross react with TRPM6 in WB.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HuT-78, JAR, and HepG2 whole cell lysate (ab7900). HeLa cells and cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4582 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109438于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 213 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 213 kDa. |
应用说明
Is unsuitable for Flow Cyt or ICC/IF.
靶标
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细胞定位
TRPM7: Membrane. TRPM6: Multi-pass membrane protein. -
数据库链接
- Entrez Gene: 140803 Human
- Entrez Gene: 54822 Human
- Omim: 605692 Human
- Omim: 607009 Human
- SwissProt: Q5VYG5 Human
- SwissProt: Q96LV9 Human
- SwissProt: Q96QT4 Human
- SwissProt: Q9BX84 Human
see all
图片
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Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : JAR cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 213 kDa -
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.cn/ps/products/109/ab109438/Images/ab109438-324289-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg)
Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)All lanes : Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/500 dilution
Lane 1 : His-Tagged human TRPM6 (aa 1755 to 2022) recombinant protein with 5% NFDM/TBST
Lane 2 : His-Tagged human TRPM7 (aa 1599 to 1986) recombinant protein with 5% NFDM/TBST
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
![Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.cn/ps/products/109/ab109438/Images/ab109438-298911-anti-trpm7-trpm6-antibody-epr4582-western-blot.jpg)
Western blot - Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438) at 1/1000 dilution (purified) + JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 213 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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![Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)](https://www.abcam.cn/ps/products/109/ab109438/Images/ab109438-6-benefits-of-recombinant-antibodies.png)
Anti-TRPM7 + TRPM6 antibody [EPR4582] (ab109438)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab109438 被引用在 15 文献中.
- Auwercx J et al. TRPM7 Modulates Human Pancreatic Stellate Cell Activation. Cells 11:N/A (2022). PubMed: 35883700
- Gao X et al. Palmitoylation regulates cellular distribution of and transmembrane Ca flux through TrpM7. Cell Calcium 106:102639 (2022). PubMed: 36027648
- Inoue H et al. The zinc-binding motif of TRPM7 acts as an oxidative stress sensor to regulate its channel activity. J Gen Physiol 153:N/A (2021). PubMed: 33999118
- Zhao Y et al. CD82-TRPM7-Numb signaling mediates age-related cognitive impairment. Geroscience 42:595-611 (2020). PubMed: 32088828
- Chen X & Song D LPS promotes the progression of sepsis by activation of lncRNA HULC/miR-204-5p/TRPM7 network in HUVECs. Biosci Rep 40:N/A (2020). PubMed: 32484206