Anti-TRPC6抗体[3F2.H10.F2] (ab105845)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)](https://www.abcam.cn/ps/products/105/ab105845/Images/ab105845-118249-anti-trpc6-antibody-3f2h10f2-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)")
Key features and details
- Mouse monoclonal [3F2.H10.F2] to TRPC6
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-TRPC6抗体[3F2.H10.F2]
参阅全部 TRPC6 一抗 -
描述
小鼠单克隆抗体[3F2.H10.F2] to TRPC6 -
宿主
Mouse -
特异性
This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
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经测试应用
适用于: WB, IHC-P, ELISAmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee
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免疫原
Synthetic peptide corresponding to a region near the C terminal of Human TRPC6 (NP_004612).
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阳性对照
- Human adrenal (cortex) tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
This product was purified from concentrated tissue culture supernate by Protein A chromatography. -
克隆
单克隆 -
克隆编号
3F2.H10.F2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105845于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 106 kDa.
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| IHC-P | (1) |
Use a concentration of 2.5 µg/ml.
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| ELISA |
1/10000 - 1/50000.
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| 说明 |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 106 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. |
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ELISA
1/10000 - 1/50000. |
靶标
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功能
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. -
组织特异性
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. -
疾病相关
Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
序列相似性
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
Contains 4 ANK repeats. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7225 Human
- Entrez Gene: 22068 Mouse
- Omim: 603652 Human
- SwissProt: Q9Y210 Human
- SwissProt: Q61143 Mouse
- Unigene: 159003 Human
- Unigene: 325086 Mouse
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别名
- bZ1P14.9 antibody
- FLJ11098 antibody
- FLJ14863 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)ab105845 at 2.5 µg/ml staining TRPC6 in Human adrenal (cortex) tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
The image shows strong staining with minimal background staining. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. -
![Western blot - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)](https://www.abcam.cn/ps/products/105/ab105845/Images/ab105845-270236-anti-trpc6-antibody-3f2h10f2-western-blot.jpg)
Western blot - Anti-TRPC6 antibody [3F2.H10.F2] (ab105845)Western blot analysis of ab105845 with mouse kidney (10ug) at the dilution 1/1000.Secondary antibody (DyLight™ 649 donkey anti-mouse) was used at the dilution 1/20000
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab105845 被引用在 1 文献中.
- Li Y et al. Downregulation of TRPC6 regulates ERK1/2 to prevent sublytic C5b‑9 complement complex‑induced podocyte injury through activating autophagy. Exp Ther Med 26:576 (2023). PubMed: 38023364