Anti-TRP2/DCT抗体(ab74073)
Key features and details
- Rabbit polyclonal to TRP2/DCT
- Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TRP2/DCT抗体
参阅全部 TRP2/DCT 一抗 -
描述
兔多克隆抗体to TRP2/DCT -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human TRP2/DCT (internal sequence).
Database link: P40126 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74073于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
|
WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF | (2) |
1/100 - 1/500.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 59 kDa. TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF
1/100 - 1/500. |
靶标
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功能
Involved in regulating eumelanin and phaeomelanin levels. -
通路
Pigment biosynthesis; melanin biosynthesis. -
序列相似性
Belongs to the tyrosinase family. -
细胞定位
Melanosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1638 Human
- Entrez Gene: 13190 Mouse
- Omim: 191275 Human
- SwissProt: P40126 Human
- SwissProt: P29812 Mouse
- Unigene: 301865 Human
- Unigene: 19987 Mouse
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别名
- L dopachrome tautomerase antibody
- DCT antibody
- Dopachrome delta isomerase antibody
see all
图片
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All lanes : Anti-TRP2/DCT antibody (ab74073) at 1/500 dilution
Lane 1 : Extracts from K562 cells with no peptide
Lane 2 : Extracts from K562 cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 117 kDa, 39 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of TRP2/DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel: no peptide
right hand panel: with immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-TRP2/DCT antibody (ab74073)This image is courtesy of an Abreview submitted by Heiko Locher
ab74073 staining TRP2/DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab74073 被引用在 57 文献中.
- Gao R et al. Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway. Front Pharmacol 14:1081030 (2023). PubMed: 36814484
- Dietsch B et al. Hepatic passaging of NRAS-mutant melanoma influences adhesive properties and metastatic pattern. BMC Cancer 23:436 (2023). PubMed: 37179302
- Stüfchen I et al. Sox9 regulates melanocytic fate decision of adult hair follicle stem cells. iScience 26:106919 (2023). PubMed: 37283806
- Rachinger N et al. Alpha-Synuclein and Its Role in Melanocytes. Cells 11:N/A (2022). PubMed: 35805172
- Tanwar J et al. MITF is a novel transcriptional regulator of the calcium sensor STIM1: Significance in physiological melanogenesis. J Biol Chem 298:102681 (2022). PubMed: 36356899