为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-TRP1抗体[TA99] (ab3312)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (29)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-TRP1 antibody [TA99] (ab3312)

    Key features and details

    • Mouse monoclonal [TA99] to TRP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2a

    你可能会感兴趣

    Western blot
    Product image
    InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
    一抗
    Product image
    Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078)
    二抗
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    查看更多相关产品

    概述

    • 产品名称

      Anti-TRP1抗体[TA99]
      参阅全部 TRP1 一抗
    • 描述

      小鼠单克隆抗体[TA99] to TRP1
    • 宿主

      Mouse
    • 特异性

      ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      SK-MEL-23 Melanoma cell line.

    • 阳性对照

      • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      TA99
    • 同种型

      IgG2a
    • 研究领域

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Proto-oncogenes
      • Cell Biology
      • Other Antibodies
      • Other Antibodies
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    相关产品

    • Alternative Versions

      • Anti-TRP1 antibody [TA99] (ab190709)
      • Anti-TRP1 antibody [TA99] - BSA and Azide free (ab212922)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human TRP1 protein (His tag) (ab219285)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3312于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    说明
    WB
    Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

    靶标

    • 功能

      Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
    • 组织特异性

      Pigment cells.
    • 通路

      Pigment biosynthesis; melanin biosynthesis.
    • 疾病相关

      Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
    • 序列相似性

      Belongs to the tyrosinase family.
    • 细胞定位

      Melanosome membrane.
    • Target information above from: UniProt accession P17643 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 7306 Human
      • Omim: 115501 Human
      • SwissProt: P17643 Human
      • Unigene: 270279 Human
      • 别名

        • 5 antibody
        • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
        • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
        • b-PROTEIN antibody
        • CAS2 antibody
        • Catalase B antibody
        • CATB antibody
        • DHICA oxidase antibody
        • Glycoprotein 75 antibody
        • GP75 antibody
        • Melanoma antigen gp75 antibody
        • OCA3 antibody
        • TRP antibody
        • TRP-1 antibody
        • TRP1 antibody
        • Tyrosinase related protein 1 antibody
        • Tyrosinase-related protein 1 antibody
        • TYRP antibody
        • TYRP1 antibody
        • TYRP1_HUMAN antibody
        • TYRRP antibody
        see all

      图片

      • Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Western blot - Anti-TRP1 antibody [TA99] (ab3312)
        Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

        Secondary
        Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 72 kDa
        Observed band size: 72 kDa
        Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.


        Exposure time: 20 minutes

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (29)

      发表研究结果有使用 ab3312?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3312 被引用在 29 文献中.

      • Aktary Z  et al. A role for Dynlt3 in melanosome movement, distribution, acidity and transfer. Commun Biol 4:423 (2021). PubMed: 33772156
      • Guo D  et al. RAB27A/Melanophilin Blocker Inhibits Melanoma Cell Motility and Invasion. J Invest Dermatol 140:1470-1473.e3 (2020). PubMed: 31978414
      • Li B  et al. Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes. Acta Pharm 70:539-549 (2020). PubMed: 32412435
      • Belote RL & Simon SM Ca2+ transients in melanocyte dendrites and dendritic spine-like structures evoked by cell-to-cell signaling. J Cell Biol 219:N/A (2020). PubMed: 31821412
      • Lv J  et al. Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway. Front Pharmacol 11:569368 (2020). PubMed: 33013408
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 15 Abreviews or Q&A

      Western blot abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Melanoma cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      Melanoma cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Aug 25 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 27 2021

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanocytes)
      Permeabilization
      No
      Specification
      Melanocytes
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 08 2021

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin - nevus)
      Permeabilization
      No
      Specification
      Skin - nevus
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 21 2020

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Melanoma cell line (SK-Mel 28))
      Permeabilization
      No
      Specification
      Melanoma cell line (SK-Mel 28)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 21 2020

      Immunocytochemistry abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (Melanoma cells)
      Permeabilization
      No
      Specification
      Melanoma cells
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      HERR DR. Christian Ostalecki

      Verified customer

      提交于 May 24 2018

      ELISA abreview for Anti-TRP1 antibody [TA99]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Serum (melanoma patient serum)
      Specification
      melanoma patient serum
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Type
      Direct
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      FRAU. Mirjam Fässler

      Verified customer

      提交于 Sep 12 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-TRP1 antibody [TA99]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
      Sample
      Human Tissue sections (Human Skin)
      Specification
      Human Skin
      Permeabilization
      Yes - 0.1% tritonx-100
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 09 2014

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRP1 antibody [TA99]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (SK-MEL-28)
      Specification
      SK-MEL-28
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Triton-X in PBS
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Oct 18 2012

      Question

      Dear Madam, Sir,

      For in vivo use we need to provide information on possible presence of murine viruses in TA99 Ab. Could you let me know if a test on murine viruses is done and what the results are.

      Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Dec 18 2012

      Answer

      Thank you for contacting us and your interest in our products.

      We have not used the anti-TRP1 antibody [TA99] ab3312 in in vivo assays and would therefore not be able to guarantee the results. I would like to point out that the antibody has 0.1% azide as a preservative which is likely to be detrimental due to its toxicity. Ab80781 is a BSA and azide free preparation of the clone TA99.

      We have not confirmed if the preparation of ab3312 or ab80781 does contain any viruses. We do not expect it would but have not confirmed this.

      I hope this information has been of help. If I can be of any further assistance, please do not hesitate to let me know.

      Read More

      Abcam Scientific Support

      回复于 Dec 18 2012

      1-10 of 15 Abreviews or Q&A

      •  向后
      • 1
      • 2
      • 向前 

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      沪ICP备14046373号-1

      沪公网安备 31011502007413号

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2023 艾博抗(上海)贸易有限公司版权所有