Anti-TRP1抗体[TA99] (ab3312)
![Western blot - Anti-TRP1 antibody [TA99] (ab3312)](https://www.abcam.cn/ps/products/3/ab3312/Images/ab3312-7437-anti-trp1-antibody-ta99-western-blot.jpg "Western blot - Anti-TRP1 antibody [TA99] (ab3312)")
Key features and details
- Mouse monoclonal [TA99] to TRP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-TRP1抗体[TA99]
参阅全部 TRP1 一抗 -
描述
小鼠单克隆抗体[TA99] to TRP1 -
宿主
Mouse -
特异性
ab3312 is specific for melanomas and does not react with carcinomas and sarcomas. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
SK-MEL-23 Melanoma cell line.
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阳性对照
- In Western Blot, this antibody gave a positive signal in human skin tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
TA99 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
靶标
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功能
Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. -
组织特异性
Pigment cells. -
通路
Pigment biosynthesis; melanin biosynthesis. -
疾病相关
Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. -
序列相似性
Belongs to the tyrosinase family. -
细胞定位
Melanosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7306 Human
- Omim: 115501 Human
- SwissProt: P17643 Human
- Unigene: 270279 Human
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别名
- 5 antibody
- 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
- 6-dihydroxyindole-2-carboxylic acid oxidase antibody
see all
图片
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Western blot - Anti-TRP1 antibody [TA99] (ab3312)Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 61 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab3312 被引用在 31 文献中.
- Wei X et al. Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF. Exp Dermatol 31:1253-1263 (2022). PubMed: 35514241
- Netcharoensirisuk P et al. Cajanin Suppresses Melanin Synthesis through Modulating MITF in Human Melanin-Producing Cells. Molecules 26:N/A (2021). PubMed: 34641584
- Aktary Z et al. A role for Dynlt3 in melanosome movement, distribution, acidity and transfer. Commun Biol 4:423 (2021). PubMed: 33772156
- Guo D et al. RAB27A/Melanophilin Blocker Inhibits Melanoma Cell Motility and Invasion. J Invest Dermatol 140:1470-1473.e3 (2020). PubMed: 31978414
- Li B et al. Exploring the potential effect and mechanisms of protocatechuic acid on human hair follicle melanocytes. Acta Pharm 70:539-549 (2020). PubMed: 32412435