AB178676

Anti-TRP1 抗体 [EPR13063]

Anti-TRP1 antibody [EPR13063]

RabMAb
Recombinant
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(21 Publications)

Rabbit Recombinant Monoclonal TRP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 21 publications.

查看别名

CAS2, TYRP, TYRRP, TYRP1, DHICA oxidase, Catalase B, Glycoprotein 75, Melanoma antigen gp75, Tyrosinase-related protein 1, TRP, TRP-1, TRP1

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (AB178676)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TRP1 with purified ab178676 at 1 : 1000 dilution (0.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (AB178676)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunocytochemistry/ Immunofluorescence analysis of B16-F10 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1 : 100 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunofluorescent analysis of B16-F0 cells labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (AB178676)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (AB178676)

Intracellular Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1/40 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Lab

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

The observed molecular weight is consistent with what have been described in literatures PMID : 24586946 and 27690000.

All lanes:

Western blot - Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution

All lanes:

SK-MEL-28 (Human malignant melanoma ) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

All lanes:

Western blot - Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution

All lanes:

Human melanoma lysate at 10 µg

Predicted band size: 61 kDa

false

Lab

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

The observed molecular weight is consistent with what have been described in literatures PMID : 24586946 and 27690000.

All lanes:

Western blot - Anti-TRP1 antibody [EPR13063] (ab178676) at 1/50000 dilution

Lane 1:

MeWo (Human malignant melanoma fibroblast) whole cell lysates at 20 µg

Lane 2:

B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 75 kDa

false

Supplier Data

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

All lanes:

Western blot - Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution

All lanes:

B16-F0 cell lysate at 10 µg

Predicted band size: 35 kDa,61 kDa,64 kDa

Observed band size: 39 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13063

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "For the unpurified format use at 1/100 - 1/250 dilution.The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

This antibody is unsuitable for IHC-P on mouse samples.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRP1 (Tyrosinase-related protein 1) also known as gp75 is a copper-binding protein involved in melanin biosynthesis. TRP1 has a molecular mass of approximately 75 kDa. It is expressed mainly in melanocytes and retinal pigment epithelium cells where it contributes to pigmentation processes. TRP1 functions as an oxidase enzyme catalyzing steps in the transformation of dopa to melanin a pigment responsible for coloration in skin hair and eyes.

Biological function summary

TRP1 participates in a protein complex known as the melanosome. In this complex it plays a role in the proper polymerization and stabilization of eumelanin one type of melanin. It influences the quality and quantity of melanin produced affecting pigmentation. TRP1’s interaction with other tyrosinase family members like tyrosinase and TRP2 is important in maintaining the complex's integrity and function with each member contributing uniquely to the melanogenesis process.

Pathways

TRP1 operates within the melanogenesis and WNT signaling pathways. In the melanogenesis pathway TRP1 works alongside tyrosinase for the production of melanin pigment. The WNT signaling pathway which is significant for cellular differentiation and proliferation connects TRP1 through regulatory networks that influence melanocyte development and pigmentation. Regulation of TRP1 and these proteins ensures proper melanin synthesis impacting pigmentation processes in the organism.

TRP1 has connections to conditions such as albinism and melanoma. In albinism mutations in TRP1 can lead to reduced melanin production causing pigmentation defects. TRP1's altered expression has been linked to melanoma a type of skin cancer highlighting its role in melanin regulation and tumor biology. The relationship between TRP1 and tyrosinase in diseases like melanoma highlights the significance of their interaction in both normal pigmentation and disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in melanin biosynthesis (PubMed : 16704458, PubMed : 22556244, PubMed : 23504663). Catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid in the presence of bound Cu(2+) ions, but not in the presence of Zn(2+) (PubMed : 28661582). May regulate or influence the type of melanin synthesized (PubMed : 16704458, PubMed : 22556244). Also to a lower extent, capable of hydroxylating tyrosine and producing melanin (By similarity).

See full target information TYRP1

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文献 (21)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Oncology 32:200862 PubMed39308793

2024

TYRP1 directed CAR T cells control tumor progression in preclinical melanoma models.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S Hackett,Daniel Hirschhorn,Meixian S Tang,Terence J Purdon,Yacine Marouf,Alessandra Piersigilli,Narasimhan P Agaram,Cailian Liu,Sara E Schad,Elisa de Stanchina,Sarwish Rafiq,Sebastien Monette,Jedd D Wolchok,Taha Merghoub,Renier J Brentjens

Nature materials 23:1736-1747 PubMed39223270

2024

Tumour-derived small extracellular vesicles act as a barrier to therapeutic nanoparticle delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Ningqiang Gong,Wenqun Zhong,Mohamad-Gabriel Alameh,Xuexiang Han,Lulu Xue,Rakan El-Mayta,Gan Zhao,Andrew E Vaughan,Zhiyuan Qin,Fengyuan Xu,Alex G Hamilton,Dongyoon Kim,Junchao Xu,Junhyong Kim,Xucong Teng,Jinghong Li,Xing-Jie Liang,Drew Weissman,Wei Guo,Michael J Mitchell

Regenerative therapy 26:203-212 PubMed38948130

2024

Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Shunxin Jin,Dapeng Cheng,Hao Chen,Yuxiang Wang,Chao Ji,Zhenzhen Yan,Xiao Fang,Shichu Xiao,Xinling Bi

Cells 13: PubMed38891124

2024

Establishment of Canine Oral Mucosal Melanoma Cell Lines and Their Xenogeneic Animal Models.

Applications

Unspecified application

Species

Unspecified reactive species

Shumin Li,Zichen Liu,Jinbao Lv,Di Lv,Huanming Xu,Hao Shi,Gang Liu,Degui Lin,Yipeng Jin

Nature communications 15:1244 PubMed38336975

2024

CAR-T cell therapy targeting surface expression of TYRP1 to treat cutaneous and rare melanoma subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Sameeha Jilani,Justin D Saco,Edurne Mugarza,Aleida Pujol-Morcillo,Jeffrey Chokry,Clement Ng,Gabriel Abril-Rodriguez,David Berger-Manerio,Ami Pant,Jane Hu,Rubi Gupta,Agustin Vega-Crespo,Ignacio Baselga-Carretero,Jia M Chen,Daniel Sanghoon Shin,Philip Scumpia,Roxana A Radu,Yvonne Chen,Antoni Ribas,Cristina Puig-Saus

International journal of molecular sciences 24: PubMed37108635

2023

Hydrolyzed Conchiolin Protein (HCP) Extracted from Pearls Antagonizes both ET-1 and -MSH for Skin Whitening.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Yang,Zhekun Wang,Yunwei Hu,Kaile Zong,Xingjiang Zhang,Hui Ke,Pan Wang,Yuyo Go,Xi Hui Felicia Chan,Jianxin Wu,Qing Huang

Cell 186:1432-1447.e17 PubMed37001503

2023

T cell immunotherapies engage neutrophils to eliminate tumor antigen escape variants.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Hirschhorn,Sadna Budhu,Lukas Kraehenbuehl,Mathieu Gigoux,David Schröder,Andrew Chow,Jacob M Ricca,Billel Gasmi,Olivier De Henau,Levi Mark B Mangarin,Yanyun Li,Linda Hamadene,Anne-Laure Flamar,Hyejin Choi,Czrina A Cortez,Cailian Liu,Aliya Holland,Sara Schad,Isabell Schulze,Allison Betof Warner,Travis J Hollmann,Arshi Arora,Katherine S Panageas,Gabrielle A Rizzuto,Rebekka Duhen,Andrew D Weinberg,Christine N Spencer,David Ng,Xue-Yan He,Jean Albrengues,David Redmond,Mikala Egeblad,Jedd D Wolchok,Taha Merghoub

Journal of cellular and molecular medicine 27:982-990 PubMed36924030

2023

Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Xie,Xingyu Mei,Weimin Shi

Science advances 8:eadd4603 PubMed36269827

2022

Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuto Kageyama,Akihiro Shimizu,Riki Anakama,Rikuma Nakajima,Kohei Suzuki,Yusuke Okubo,Junji Fukuda

Molecules (Basel, Switzerland) 27: PubMed36235295

2022

Molecular Analysis of the Melanogenesis Inhibitory Effect of Saponins-Rich Fraction of Leaves Extract.

Applications

Unspecified application

Species

Unspecified reactive species

Myra O Villareal,Thanyanan Chaochaiphat,Rachida Makbal,Chemseddoha Gadhi,Hiroko Isoda

View all publications

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Anti-TRP1 抗体 [EPR13063]

Anti-TRP1 antibody [EPR13063]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (AB178676)

Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (AB178676)

Flow Cytometry (Intracellular) - Anti-TRP1 antibody [EPR13063] (AB178676)

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

Western blot - Anti-TRP1 antibody [EPR13063] (AB178676)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性