AB207416

重组Anti-TROVE2/SS-A抗体[EPR20322]

Anti-TROVE2/SS-A antibody [EPR20322]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal TROVE2/SS-A antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

查看别名

SSA2, TROVE2, RO60, RNA-binding protein RO60, 60 kDa SS-A/Ro ribonucleoprotein, Ro 60 kDa autoantigen, Sjoegren syndrome antigen A2, Sjoegren syndrome type A antigen, TROVE domain family member 2, 60 kDa Ro protein, 60 kDa ribonucleoprotein Ro, RoRNP, Ro60 autoantigen, SS-A

4 Images

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (AB207416)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/10000 dilution

Lane 1:

MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 3:

293T (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (AB207416)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Lane 4:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (AB207416)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (AB207416)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-7 : 30 seconds; Lane 8 : 3 minutes.

All lanes:

Western blot - Anti-TROVE2/SS-A antibody [EPR20322] (ab207416) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Lane 4:

Rat spleen lysate at 10 µg

Lane 5:

Mouse brain lysate at 10 µg

Lane 6:

Mouse kidney lysate at 10 µg

Lane 7:

Mouse spleen lysate at 10 µg

Lane 8:

Mouse heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-TROVE2/SS-A antibody [EPR20322] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20322

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TROVE2 also known as SS-A or Ro60 is a ribonucleoprotein with molecular mass approximately 60 kDa. The protein is present mainly in the cytoplasm and nucleus of eukaryotic cells. TROVE2 functions as an essential part of the Ro ribonucleoprotein complex which binds non-coding RNA to protect it from degradation. This protein plays a role in recognizing misfolded RNA molecules and assists in their correct folding or degradation ensuring RNA quality control in the cell.

Biological function summary

TROVE2 is involved in several important cellular processes. The protein often exists as part of a complex together with La protein and small Y RNAs contributing to RNA biogenesis and stability. It helps regulate the cellular stress response by acting on misfolded RNAs and interacts with various RNA molecules through specific binding mechanisms. TROVE2's role in maintaining RNA integrity suggests it has a wider impact on the stability of the transcriptome.

Pathways

The protein TROVE2 integrates into immune response and RNA quality control pathways. It associates with molecules involved in RNA surveillance like the La protein and modulates the cell's reaction to environmental stress and pathogenic challenges. TROVE2 influences the pathways connected to RNA processing and degradation therefore intersecting with networks ensuring proper cellular function and stress management.

TROVE2 shows a connection with autoimmune disorders such as Sjögren's syndrome and systemic lupus erythematosus (SLE). These diseases often feature the production of autoantibodies against TROVE2 associating it with immune dysregulation. The interplay between TROVE2 and the La protein is notable in these conditions as both participate in producing autoantibodies contributing to the pathology of these autoimmune syndromes.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs (PubMed : 18056422, PubMed : 26382853). Binds to endogenous Alu retroelements which are induced by type I interferon and stimulate porinflammatory cytokine secretion (PubMed : 26382853). Regulates the expression of Alu retroelements as well as inflammatory genes (PubMed : 26382853). May play roles in cilia formation and/or maintenance (By similarity).

See full target information RO60

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 9:1293-1311 PubMed38622380

2024

Age-specific nasal epithelial responses to SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Maximillian N J Woodall,Ana-Maria Cujba,Kaylee B Worlock,Katie-Marie Case,Tereza Masonou,Masahiro Yoshida,Krzysztof Polanski,Ni Huang,Rik G H Lindeboom,Lira Mamanova,Liam Bolt,Laura Richardson,Batuhan Cakir,Samuel Ellis,Machaela Palor,Thomas Burgoyne,Andreia Pinto,Dale Moulding,Timothy D McHugh,Aarash Saleh,Eliz Kilich,Puja Mehta,Chris O'Callaghan,Jie Zhou,Wendy Barclay,Paolo De Coppi,Colin R Butler,Mario Cortina-Borja,Heloise Vinette,Sunando Roy,Judith Breuer,Rachel C Chambers,Wendy E Heywood,Kevin Mills,Robert E Hynds,Sarah A Teichmann,Kerstin B Meyer,Marko Z Nikolić,Claire M Smith

Cell genomics 3:100303 PubMed37228754

2023

exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Emily L LaPlante,Alessandra Stürchler,Robert Fullem,David Chen,Anne C Starner,Emmanuel Esquivel,Eric Alsop,Andrew R Jackson,Ionita Ghiran,Getulio Pereira,Joel Rozowsky,Justin Chang,Mark B Gerstein,Roger P Alexander,Matthew E Roth,Jeffrey L Franklin,Robert J Coffey,Robert L Raffai,Isabelle M Mansuy,Stavros Stavrakis,Andrew J deMello,Louise C Laurent,Yi-Ting Wang,Chia-Feng Tsai,Tao Liu,Jennifer Jones,Kendall Van Keuren-Jensen,Eric Van Nostrand,Bogdan Mateescu,Aleksandar Milosavljevic

View all publications

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重组Anti-TROVE2/SS-A抗体[EPR20322]