AB250756

重组Anti-Troponin I fast skeletal muscle抗体[EPR14927(B)] - BSA and Azide free

Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Troponin I fast skeletal muscle antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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TNNI2

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Supplier Data

Immunoprecipitation - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free (AB250756)

This data was developed using ab184554, the same antibody clone in a different buffer formulation.

Western blot analysis of Troponin I fast skeletal muscle in Human skeletal muscle lysate immunoprecipitated using ab184554 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Human skeletal muscle lysate.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (<a href='/products/primary-antibodies/troponin-i-fast-skeletal-muscle-antibody-epr14927b-ab184554'>ab184554</a>)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free (AB250756)

This data was developed using ab184554, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] (<a href='/products/primary-antibodies/troponin-i-fast-skeletal-muscle-antibody-epr14927b-ab184554'>ab184554</a>) at 1/20000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Supplier Data

Western blot - Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)] - BSA and Azide free (AB250756)

This data was developed using ab184554, the same antibody clone in a different buffer formulation.

All lanes:

Lane 1:

Mouse muscle lysate at 10 µg

Lane 2:

Rat muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-Troponin I fast skeletal muscle antibody [EPR14927(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14927(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab250756 is the carrier-free version of ab184554.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Troponin I fast skeletal muscle also known as TNNI2 is a protein involved in muscle contraction. It weighs around 21.2 kDa and is mainly expressed in fast-twitch skeletal muscle. TNNI2 acts by inhibiting actomyosin ATPase activity meaning it helps prevent muscle contraction until it receives a signal to contract. It binds to actin and plays a regulatory role in the muscle contraction process.

Biological function summary

Troponin I fast skeletal muscle interacts within the troponin complex which includes Troponin T and Troponin C. Located on the thin filament of the muscle fiber this complex modulates the contraction of striated muscle by controlling calcium binding. When calcium binds to Troponin C it induces a conformational change that displaces TNNI2 from actin allowing for muscle contraction to occur.

Pathways

Troponin I fast skeletal muscle has essential functions in the calcium modulation pathways specific to muscle contraction. It plays an important role in the regulation of muscle contraction by interacting with calcium ions and other proteins such as tropomyosin. As part of the excitation-contraction coupling pathway TNNI2 helps ensure the proper contraction of muscles in response to neural stimulation. Additionally its role in these pathways involves specific interactions with proteins like Troponin C and Troponin T which are necessary for functional muscle contraction.

Abnormalities in Troponin I fast skeletal muscle can lead to muscle-related conditions such as nemaline myopathy and distal arthrogryposis. These disorders affect muscle structure and function leading to muscle weakness or deformities. Mutations in TNNI2 may result in improper interaction with other sarcomeric proteins such as tropomyosin disrupting normal muscle contraction and leading to the mentioned diseases. Understanding the role of TNNI2 in these disorders can aid in the development of diagnostic and therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI2

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重组Anti-Troponin I fast skeletal muscle抗体[EPR14927(B)] - BSA and Azide free