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Anti-Troponin I fast skeletal muscle抗体[2F12G2] (ab126824)

Submit a review Q&A (2)References (1)
Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
  • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
  • - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
  • Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
  • Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)

Key features and details

  • Mouse monoclonal [2F12G2] to Troponin I fast skeletal muscle
  • Suitable for: WB, ELISA, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Troponin I fast skeletal muscle抗体[2F12G2]
    参阅全部 Troponin I fast skeletal muscle 一抗

  • 描述

    小鼠单克隆抗体[2F12G2] to Troponin I fast skeletal muscle

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Purified full-length recombinant Human Troponin I fast skeletal muscle expressed in E. Coli.

  • 阳性对照

    • Recombinant Human TNNI2 protein (AA: 1-182), HEK293 and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysates.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein G purified

  • 纯化说明

    Purified from tissue culture supernatant.

  • 克隆

    单克隆

  • 克隆编号

    2F12G2

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126824于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.
说明
WB
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA
1/10000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/1000.

靶标

  • 功能

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

  • 疾病相关

    Defects in TNNI2 are a cause of distal arthrogryposis type 2B (DA2B) [MIM:601680]; also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin.

  • 序列相似性

    Belongs to the troponin I family.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AMCD 2B antibody
      • AMCD2B antibody
      • DA 2B antibody
      • DA2B antibody
      • fast skeletal muscle antibody
      • Fast twitch skeletal muscle troponin I antibody
      • fast-twitch isoform antibody
      • FSSV antibody
      • fsTnI antibody
      • TNNI 2 antibody
      • Tnni2 antibody
      • TNNI2_HUMAN antibody
      • tro antibody
      • Troponin I antibody
      • Troponin I fast skeletal muscle antibody
      • Troponin I fast twitch 2 antibody
      • Troponin I fast twitch isoform antibody
      • Troponin I fast twitch skeletal muscle isoform antibody
      • Troponin I skeletal fast antibody
      • Troponin I type 2 (skeletal fast) antibody
      • Troponin I type 2 antibody
      see all

    图片

    • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution + Recombinant Human TNNI2 protein (AA: 1-182)

      Predicted band size: 21 kDa

    • Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Western blot - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      All lanes : Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824) at 1/500 dilution

      Lane 1 : HEK293 cell lysate
      Lane 2 : TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 cell lysate

      Predicted band size: 21 kDa

    • - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Example of standard curves obtained using ab126824. Control antigen 100ng (black), antigen 10ng (purple), antigen 50ng (blue), antigen 100ng (red)
    • Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Immunocytochemistry/ Immunofluorescence - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Immunofluorescence analysis of Hela cells using ab126824 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
    • Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Flow Cytometry - Anti-Troponin I fast skeletal muscle antibody [2F12G2] (ab126824)
      Flow cytometric analysis of NIH/3T3 cells using ab126824 AT 1/200 (green) and negative control (red).

    文献 (1)

    发表研究结果有使用 ab126824？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab126824 被引用在 1 文献中.

    • Boularaoui SM  et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med 12:e918-e936 (2018). PubMed: 28101909

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    The antibody ab126824 is failing in sandwich ELISA in combination with antibody ab123876, raised against the N terminus. What is the immunogen for ab126824, and where is the epitope located?

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    Answer

    Thank you for contacting us.

    I have confirmed that the immunogen for ab126824 is full-length recombinant protein, not a fragment. The epitope has not been mapped. The trouble you are having with the sandwich ELISA may be a consequence of the two antibodies recognizing overlapping epitopes but it is more likely that one of the antibodies is failing. If you can, try checking for this with an indirect ELISA against antigen coated directly to the plate, followed by blocking with BSA, the antibody, and a secondary conjugated antibody.

    For the sandwich, I recommend capturing with the monoclonal and detecting with the polyclonal ab123876, assuming you have an anti-rabbit secondary. Ideally, it should be absorbed to remove reactivity with mouse IgG1, the isotype of ab126824.

    I hope this is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    What is the concentration of this antibody?

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    Answer

    Thank you for your inquiry.


    The antibody’s concentration is 1 mg/ml.


    I hope this information helps. Please contact us with any other questions.

    Read More

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