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Anti-Tropomyosin抗体[TM-311] (ab7785)

Reviews (1)Q&A (1)References (16)
Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)

    Key features and details

    • Mouse monoclonal [TM-311] to Tropomyosin
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-Tropomyosin抗体[TM-311]

    • 描述

      小鼠单克隆抗体[TM-311] to Tropomyosin

    • 宿主

      Mouse

    • 特异性

      This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Purified chicken gizzard tropomyosin.

    • 阳性对照

      • WB: Human heart tissue lysate.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...

    • 纯度

      Protein G purified

    • 克隆

      单克隆

    • 克隆编号

      TM-311

    • 同种型

      IgG1

    • 轻链类型

      kappa

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7785于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).
    说明
    WB
    1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).

    靶标

    • 相关性

      Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.

    • 细胞定位

      Cytoplasmic

    • 数据库链接

      see all

    • 别名

      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    图片

    • Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)
      Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785)
      Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 33 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?

    文献 (16)

    发表研究结果有使用 ab7785？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab7785 被引用在 16 文献中.

    • Sultan A  et al. Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat. Life (Basel) 12:N/A (2022). PubMed: 36013400
    • Zhang W  et al. Skullcapflavone I inhibits proliferation of human colorectal cancer cells via down-regulation of miR-107 expression. Neoplasma 66:203-210 (2019). PubMed: 30509103
    • Shibata T  et al. Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev 171:24-30 (2018). IHC-P ; Mouse . PubMed: 29510160
    • Sui Z  et al. Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells. Mol Biol Cell 28:2531-2542 (2017). PubMed: 28720661
    • Ijaz F  et al. Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4. Mol Biol Cell 28:535-544 (2017). PubMed: 27974641
    View all Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Tropomyosin antibody [TM-311]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Myometrium)
    Loading amount
    20 µg
    Specification
    Myometrium
    Gel Running Conditions
    Reduced Denaturing (gel 12%,)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 14 2011

    Question

    Which isotype(s) does this antibody recognize? Does it recognize the beta isoform? What exactly does it mean by "This antibody is specific for the 36K and 39K bands of chicken gizzard tropomyosin?"

    Read More
    Answer

    Thank you for your phone call. The originator of ab7785 provided me with the following information regarding this antibody. The molecular weight of tropomyosin is 70 kDa. The alpha isomer is 36 kDa and the beta isomer is 35 kDa. In electrophoresis one gets two bands and they should be 35 kDa and 36 kDa. In the originator's hands (with the chicken gizzard samples), they got two bands: the beta isoform running at 36 kDa and the alpha isoform running at 39 kDa. So this antibody recognizes the alpha and beta isoforms. If you have any additional questions, please do let me know.

    Read More

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