重组Anti-TROP2抗体[EPR20043] - BSA and Azide free (ab271996)
![Western blot - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-522356-anti-trop2-antibody-epr20043-western-blot-wildtype-mcf7-tacstd2-knockout-mcf7-hct-116-skbr3-human.jpg "Western blot - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20043] to TROP2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TROP2抗体[EPR20043] - BSA and Azide free
参阅全部 TROP2 一抗 -
描述
兔单克隆抗体[EPR20043] to TROP2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human skin, breast and cervix cancer tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HCT 116 cells. Flow Cyt (intra): MCF7 cells. WB: HCT 116, PC-3 and MCF7 whole cell lysates; human breast cancer, skin, placenta and prostate cancer lysates, Mouse and rat skin and lung lysate, mouse kidney lysate.
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常规说明
ab271996 is the carrier-free version of ab214488.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20043 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab214488 is recommended for mouse and rat in IHC
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab214488 is recommended for mouse and rat in IHC
|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
靶标
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功能
May function as a growth factor receptor. -
组织特异性
Placenta, pancreatic carcinoma cell lines. -
疾病相关
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
序列相似性
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4070 Human
- Entrez Gene: 56753 Mouse
- Entrez Gene: 494343 Rat
- Omim: 137290 Human
- SwissProt: P09758 Human
- SwissProt: Q8BGV3 Mouse
- SwissProt: Q6P9Z6 Rat
- Unigene: 23582 Human
see all -
别名
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
图片
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Western blot - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : TACSTD2 knockout MCF7 cell lysate
Lane 3 : HCT 116 cell lysate
Lane 4 : SK-BR-3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36-70 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
Western blot: Anti-TACSTD2 antibody [EPR20043] (ab214488) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab214488 was shown to bind specifically to TACSTD2. A band was observed at 36-70 kDa in wild-type MCF7 cell lysates with no signal observed at this size in TACSTD2 knockout cell line. To generate this image, wild-type and TACSTD2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.|
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![Western blot - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-512002-anti-trop2-antibody-epr20043-western-blot-tissue-lysate-mouse-rat.jpg)
Western blot - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 1 : Mouse skin lysate at 20 µg
Lane 2 : Mouse kidney lysate at 20 µg
Lane 3 : Mouse lung lysate at 20 µg
Lane 4 : Rat skin lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Exposure time: 60 secondsThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
ab181602 was used as a GAPDH loading control.
Negative sample: A549 (PMID: 22419550).
TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-495840-anti-trop2-epr20043-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling TACD2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of rat skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-7-ab214488266897antitrop2antibodyepr20043immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of human skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-6-ab214488266896antitrop2antibodyepr20043immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the duct epithelium of human breast is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-5-ab214488266895antitrop2antibodyepr20043immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the tumor cells of human cervix cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-4-ab214488266894antitrop2antibodyepr20043immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Membrane staining on the squamous epithelium of mouse skin is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488). -
![Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-3-ab214488266892antitrop2antibodyepr20043immunohistochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane and weakly cytoplasmic staining on MCF7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488). -
![Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-2-ab214488266891antitrop2antibodyepr20043immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing membrane staining on HCT 116 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-1-ab214488266890antitrop2antibodyepr20043flowcytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/60 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
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![Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)](https://www.abcam.cn/ps/products/271/ab271996/Images/ab271996-8-benefits-of-recombinant-antibodies.png)
Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
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