Name: Anti-TROP2 antibody [EPR20043]
Brand: RabMAb
SKU: ab214488
Rating: 5 (9 reviews)

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Immunocytochemistry - Anti-TROP2 antibody [EPR20043] (ab214488)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20043] to TROP2
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free Carrier Free HRP PE

产品名称

Anti-TROP2抗体[EPR20043]
参阅全部 TROP2 一抗
描述

兔单克隆抗体[EPR20043] to TROP2
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HCT 116, PC-3 and MCF7 whole cell lysates; human breast cancer, skin, placenta and prostate cancer lysates, Mouse and rat skin and lung lysate, mouse kidney lysate. IHC-P: Human skin, breast and cervix cancer tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HCT 116 cells. Flow Cyt (intra): MCF7 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR20043
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human TACSTD2 knockout MCF7 cell line (ab286330)
Recombinant Protein
- Recombinant Human TROP2 protein (ab114374)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Abpromise™承诺保证使用ab214488于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(1)	1/2000. Detects a band of approximately 37-50 kDa (predicted molecular weight: 36 kDa).
ICC/IF	(1)	1/100.
IHC-P	(4)	1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab214488 is recommended for mouse and rat in IHC

说明
Flow Cyt (Intra) 1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/2000. Detects a band of approximately 37-50 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/100.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab214488 is recommended for mouse and rat in IHC

功能

May function as a growth factor receptor.
组织特异性

Placenta, pancreatic carcinoma cell lines.
疾病相关

Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
序列相似性

Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain.
翻译后修饰

The N-terminus is blocked.
细胞定位

Membrane.
Target information above from: UniProt accession P09758 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 4070 Human
- Entrez Gene: 56753 Mouse
- Entrez Gene: 494343 Rat
- Omim: 137290 Human
- SwissProt: P09758 Human
- SwissProt: Q8BGV3 Mouse
- SwissProt: Q6P9Z6 Rat
- Unigene: 23582 Human
- Unigene: 439913 Mouse
- Unigene: 24809 Rat
see all
别名
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
- Epithelial glycoprotein 1 antibody
- GA733 1 antibody
- GA7331 antibody
- M1S 1 antibody
- M1S1 antibody
- Membrane component chromosome 1 surface marker 1 antibody
- Pancreatic carcinoma marker protein GA733 1 antibody
- Pancreatic carcinoma marker protein GA733-1 antibody
- Pancreatic carcinoma marker protein GA7331 antibody
- TACD 2 antibody
- TACD2_HUMAN antibody
- TACSTD 2 antibody
- Tacstd2 antibody
- Trop 2 antibody
- Trop2 antibody
- Tumor associated calcium signal transducer 2 precursor antibody
- Tumor-associated calcium signal transducer 2 antibody
see all

Immunocytochemistry - Anti-TROP2 antibody [EPR20043] (ab214488)This image is courtesy of an anonymous Abreview

Immunocytochemistry analysis of acetone-fixed human keratinocytes staining with ab214488 at 1/500 dilution. Secondary antibody was Alexa Fluor® 488 Goat anti-rabbit-IgG at 1/500 dilution. Samples were incubated with the primary antibody with PBS for 1 hour at 21°C. Blocking was done using 4% serum for 30 minutes at 21°C
See Abreview
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution

Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : TACSTD2 knockout MCF7 cell lysate
Lane 3 : HCT 116 cell lysate
Lane 4 : SK-BR-3 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 36 kDa
Observed band size: 36-70 kDa why is the actual band size different from the predicted?

Western blot: Anti-TACSTD2 antibody [EPR20043] (ab214488) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab214488 was shown to bind specifically to TACSTD2. A band was observed at 36-70 kDa in wild-type MCF7 cell lysates with no signal observed at this size in TACSTD2 knockout cell line. To generate this image, wild-type and TACSTD2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.|
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution

Lane 1 : Mouse skin lysate at 20 µg
Lane 2 : Mouse kidney lysate at 20 µg
Lane 3 : Mouse lung lysate at 20 µg
Lane 4 : Rat skin lysate at 20 µg
Lane 5 : Rat lung lysate at 20 µg
Lane 6 : A549 (Human lung carcinoma epithelial cell) whole cell lysate

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 36 kDa

Exposure time: 60 seconds

Blocking and diluting buffer and concentration: 5% NFDM /TBST.

ab181602 was used as a GAPDH loading control.

Negative sample: A549 (PMID: 22419550).
TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813).
Flow Cytometry (Intracellular) - Anti-TROP2 antibody [EPR20043] (ab214488)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/60 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody. The permeabilization step was done using 90% methanol for 30 mins at -20 degrees.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the tumor cells of human cervix cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane and weakly cytoplasmic staining on MCF7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution

Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 37-50 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/3: 2 seconds; Lane 2: 1 minute.

TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the duct epithelium of human breast is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

Lanes 1-3 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 4 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/5000 dilution

Lane 1 : Human breast cancer lysate
Lane 2 : Human skin lysate
Lane 3 : Human placenta lysate
Lane 4 : Human prostate cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 36 kDa
Observed band size: 37-50 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/3: 3 seconds; Lane 2: 5 seconds; Lane 4: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the squamous epithelium of human skin is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling TACD2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the squamous epithelium of rat skin is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the squamous epithelium of mouse skin is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-TROP2 antibody [EPR20043] (ab214488)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane staining on HCT 116 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols
Protocol Booklet

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab214488？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab214488 被引用在 19 文献中.

Terzic J et al. Hypoxia-inducible factor 1A inhibition overcomes castration resistance of prostate tumors. EMBO Mol Med 15:e17209 (2023). PubMed: 37070472
Liatsou E et al. The Impact of Trophoblast Cell-Surface Antigen 2 Expression on the Survival of Patients with Gastrointestinal Tumors: A Systematic Review. J Pers Med 13:N/A (2023). PubMed: 37888056
Ito T et al. TROP2 Expression in Sebaceous and Sweat Gland Carcinoma. J Clin Med 11:N/A (2022). PubMed: 35160059
Mikuteit M et al. Trophoblast Cell Surface Antigen 2 (Trop2) in Chromophobe Renal Cell Carcinoma. Oncology 100:331-336 (2022). PubMed: 35168242
Fan Y et al. Head-to-Head Comparison of the Expression Differences of NECTIN-4, TROP-2, and HER2 in Urothelial Carcinoma and Its Histologic Variants. Front Oncol 12:858865 (2022). PubMed: 35515131

View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

Skin cancer

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 17 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 31 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 31 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C

Fixative

10% NBF

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 31 2018

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

KO cell lines

Recombinant Protein

Related Products

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (19)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TROP2 antibody [EPR20043]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TROP2 antibody [EPR20043]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TROP2 antibody [EPR20043]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TROP2 antibody [EPR20043]