Anti-TROP2抗体[162-46.2] (ab79976)
![Flow Cytometry (Intracellular) - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.cn/ps/products/79/ab79976/Images/ab79976-296165-anti-trop2-antibody-162-462-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-TROP2 antibody [162-46.2] (ab79976)")
Key features and details
- Mouse monoclonal [162-46.2] to TROP2
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-TROP2抗体[162-46.2]
参阅全部 TROP2 一抗 -
描述
小鼠单克隆抗体[162-46.2] to TROP2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human TROP2. Human choriocarcinoma cell line BeWo.
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阳性对照
- IHC-P: Human placenta tissue. ICC/IF: JEG3 and MCF7 cells. Flow Cyt (Intra): JEG3 and MCF7 cells.
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常规说明
Isotype: IgG1 (Igh-4a allotype)
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
162-46.2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79976于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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Flow Cyt (Intra)
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
May function as a growth factor receptor. -
组织特异性
Placenta, pancreatic carcinoma cell lines. -
疾病相关
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
序列相似性
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4070 Human
- Omim: 137290 Human
- SwissProt: P09758 Human
- Unigene: 23582 Human
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别名
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-TROP2 antibody [162-46.2] (ab79976)
Overlay histogram showing JEG3 cells stained with ab79976 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab79976, 0.1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (monoclonal) (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in JEG3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.cn/ps/products/79/ab79976/Images/ab79976-8-anti-trop2-16246-2-antibody-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [162-46.2] (ab79976)ab79976 staining TROP2 in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab79976 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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![Flow Cytometry (Intracellular) - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.cn/ps/products/79/ab79976/Images/ab79976-95364-anti-trop2-antibody-162-462-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-TROP2 antibody [162-46.2] (ab79976)Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [162-46.2] (ab79976)](https://www.abcam.cn/ps/products/79/ab79976/Images/ab79976-95366-anti-trop2-antibody-162-462-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [162-46.2] (ab79976)ab79976 staining TROP2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab79976 被引用在 4 文献中.
- Grünherz L et al. Osteoidosis leads to altered differentiation and function of osteoclasts. J Cell Mol Med 24:5665-5674 (2020). PubMed: 32283567
- Im H et al. Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone. Proc Natl Acad Sci U S A 112:5613-8 (2015). PubMed: 25870273
- Viswanatha R et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). ICC/IF ; Human . PubMed: 24151071
- Lipinski M et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A 78:5147-50 (1981). PubMed: 7029529