Anti-TRNT1抗体(ab224536)
Key features and details
- Rabbit polyclonal to TRNT1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TRNT1抗体 -
描述
兔多克隆抗体to TRNT1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human TRNT1 aa 300-400.
Database link: Q96Q11 -
阳性对照
- WB: NIH/3T3, NBT-II and A549 cell lysates. IHC-P: Human stomach tissue. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224536于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
TRNT1 belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. It adds and repairs the conserved 3'-CCA sequence necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. -
细胞定位
Mitochondrial -
数据库链接
- Entrez Gene: 51095 Human
- Entrez Gene: 70047 Mouse
- Entrez Gene: 312616 Rat
- Omim: 612907 Human
- SwissProt: Q96Q11 Human
- SwissProt: Q8K1J6 Mouse
- SwissProt: Q4VBH2 Rat
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别名
- AA408384 antibody
- C76540 antibody
- CCA adding antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TRNT1 antibody (ab224536)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TRNT1 (green) using ab224536 at 4 µg/ml in ICC/IF.
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Western blot - Anti-TRNT1 antibody (ab224536)Anti-TRNT1 antibody (ab224536) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRNT1 antibody (ab224536)Paraffin-embedded human stomach tissue stained for TRNT1 using ab224536 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-TRNT1 antibody (ab224536)All lanes : Anti-TRNT1 antibody (ab224536) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 50 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224536 被引用在 1 文献中.
- Wei CJ et al. Case report: Muscle involvement in a Chinese patient with TRNT1-related disorder. Front Pediatr 11:1160107 (2023). PubMed: 37215601