Anti-TRMT12抗体(ab113103)
Key features and details
- Rabbit polyclonal to TRMT12
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRMT12抗体 -
描述
兔多克隆抗体to TRMT12 -
宿主
Rabbit -
特异性
ab113103 is predicted to not cross-react with other TRMT protein family members. At least two isoforms of TRMT12 are known to exist, ab113013 will detect both isoforms. -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide containing a 19 amino acid sequence from the N terminal region of Human TRMT12 (Q53H54).
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阳性对照
- K562 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab113103 is purified by affinity chromatography via a peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa.
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| ICC |
Use a concentration of 10 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa. |
|
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. -
通路
tRNA modification; wybutosine-tRNA(Phe) biosynthesis. -
序列相似性
Belongs to the RNA methyltransferase trmD family. TYW2 subfamily. - Information by UniProt
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数据库链接
- Entrez Gene: 55039 Human
- Omim: 611244 Human
- SwissProt: Q53H54 Human
- Unigene: 9925 Human
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别名
- Alpha amino alpha carboxypropyl transferase TYW2 antibody
- FLJ20772 antibody
- Homolog of yeast tRNA methyltransferase antibody
see all
图片
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Western blot - Anti-TRMT12 antibody (ab113103)All lanes : Anti-TRMT12 antibody (ab113103) at 0.5 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 50 kDa -
Immunocytochemistry - Anti-TRMT12 antibody (ab113103)Immunocytochemical analysis of K562 cells, staining TRMT12 with ab113103 at 10 µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab113103 尚未被引用在任何文献中。