重组Anti-TrkC抗体[EPR22959-40]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded HEK-293T tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with TrkC expression vector. No staining on (B) HEK-293T transfected with a TrkA expression vector, (C) HEK-293T transfected with TrkB expression vector and (D) HEK-293T transfected with empty vector. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Panel A : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with TrkC expression vector containing a myc-His-tag®
Panel B : HEK-293T transfected with TrkA vector containing a myc-His-tag®.
Panel C : HEK-293T transfected with TrkB vector containing a myc-His-tag®.
Panel D : HEK-293T transfected with empty vector containing a myc-His-tag®

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : No staining in human liver. The section was incubated with ab240651 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human cerebrum. The section was incubated with ab240651 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TrkC with ab240651 at 1/200 (2.645 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human astrocytoma. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on myenteric plexus of rat colon. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkC antibody [EPR22959-40] (AB240651)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling TrkC with ab240651 at 1/1000 (0.529 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse cerebrum. The section was incubated with ab240651 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-TrkC antibody [EPR22959-40] (AB240651)

TrkC was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab240651 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240651 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab240651 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab240651 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-TrkC antibody [EPR22959-40] (ab240651)

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-TrkC antibody [EPR22959-40] (AB240651)

TrkC was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab240651 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240651 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab240651 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab240651 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

All lanes:

Immunoprecipitation - Anti-TrkC antibody [EPR22959-40] (ab240651)

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• WB

Lab

Western blot - Anti-TrkC antibody [EPR22959-40] (AB240651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW are consistent with what has been described in the literature (PMID : 17828769).

Exposure time : 8 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hypothalamus tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• WB

Lab

Western blot - Anti-TrkC antibody [EPR22959-40] (AB240651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW are consistent with what has been described in the literature (PMID : 17828769).

Negative control : liver (PMID : 7823156).

Exposure time : 15 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution

Lane 1:

Human hypothalamus tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• WB

Lab

Western blot - Anti-TrkC antibody [EPR22959-40] (AB240651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW are consistent with what has been described in the literature (PMID : 17828769).

Negative control : liver (PMID : 7823156).

Exposure time : 37 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 40 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• WB

Lab

Western blot - Anti-TrkC antibody [EPR22959-40] (AB240651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW are consistent with what has been described in the literature (PMID : 17828769).

Exposure time : 37 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 100 kDa,145 kDa

false

• WB

Lab

Western blot - Anti-TrkC antibody [EPR22959-40] (AB240651)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody does not cross-react with human TrkA and TrkB.

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-TrkC antibody [EPR22959-40] (ab240651) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control) containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with human TrkC expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with human TrkA expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human TrkB expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 94 kDa

Observed band size: 145 kDa

false