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重组RabMAb

重组Anti-TrkB抗体[EPR17805-146] - Low endotoxin，Azide free (ab223155)

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Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
  • Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17805-146] to TrkB - Low endotoxin, Azide free
  • Suitable for: Indirect ELISA, WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat

概述

  • 产品名称

    Anti-TrkB抗体[EPR17805-146] - Low endotoxin，Azide free
    参阅全部 TrkB 一抗

  • 描述

    兔单克隆抗体[EPR17805-146] to TrkB - Low endotoxin，Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Indirect ELISA, WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Mouse TrkB active protein; mouse and rat brain lysates. IHC-P: Mouse and rat cerebral cortex tissues and mouse primary neuron cell. IP: Mouse brain lysate. ELISA: Mouse TrkB antigen.

  • 常规说明

    ab223155 is the carrier-free version of ab187041.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223155于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Indirect ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
说明
Indirect ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

  • 组织特异性

    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Ligand-mediated auto-phosphorylation.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic receptor tyrosine kinase 2 antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    图片

    • Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Indirect ELISA - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      This data was developed using ab223155, the same antibody clone in a different buffer formulation.

      ELISA analysis of Mouse TrkB (Ntrk2) recombinant protein at 1000 ng/ml with ab187041. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      This data was developed using ab187041, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling TrkB with ab187041 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic and membrane staining on mouse cerebral cortex is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasmic and membrane staining on rat cerebral cortex is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling TrkB with ab187041 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative control: no staining on mouse liver [PMID: 2555172].

      Counter stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Immunoprecipitation - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

      TrkB was immunoprecipitated from 1 mg of mouse brain lysate with ab187041 at 1/50 dilution.

      Western blot was performed from the immunoprecipitate using ab187041 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: Mouse brain lysate 10µg (Input).

      Lane 2: ab187041 IP in mouse brain lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab187041 in mouse brain lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187041).

    • Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)
      Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (ab223155)

    文献 (0)

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