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Anti-Tristetraprolin/TTP抗体[OTI8B5] - BSA and Azide free (ab273646)

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Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Flow Cytometry (Intracellular) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

Key features and details

  • Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Dog, Human, African green monkey
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Tristetraprolin/TTP抗体[OTI8B5] - BSA and Azide free
    参阅全部 Tristetraprolin/TTP 一抗

  • 描述

    小鼠单克隆抗体[OTI8B5] to Tristetraprolin/TTP - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Dog, Human, African green monkey

  • 免疫原

    Recombinant full length protein corresponding to Human Tristetraprolin/TTP aa 1-350.
    Database link: P26651

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: HeLa, SVT2, A549, COS7, Jurkat, MDCK and MCF7 cells lysate; IHC-P: Human colon, colon adenocarcinoma, ovary, endometrium, prostate tissue; IF: COS7 cell lysate; Flow Cyt (Intra): HEK-293T transfected with Tristetraprolin

  • 常规说明

    ab273646 is the carrier-free version of ab124024.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273646于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.
WB
1/1000. Predicted molecular weight: 34 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.
说明
Flow Cyt (Intra)
1/100.
WB
1/1000. Predicted molecular weight: 34 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100.

靶标

  • 功能

    mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).

  • 序列相似性

    Contains 2 C3H1-type zinc fingers.

  • 翻译后修饰

    Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.

  • 细胞定位

    Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt

  • 数据库链接

    • 别名

      • G0/G1 switch regulatory protein 24 antibody
      • G0S24 antibody
      • GOS24 antibody
      • Growth factor-inducible nuclear protein NUP475 antibody
      • NUP475 antibody
      • Protein TIS11A antibody
      • RNF162A antibody
      • TIS 11 antibody
      • TIS11 antibody
      • TIS11A antibody
      • Tristetraprolin antibody
      • Tristetraproline antibody
      • TTP antibody
      • TTP_HUMAN antibody
      • Zfp-36 antibody
      • ZFP36 antibody
      • Zinc finger protein 36 antibody
      • Zinc finger protein 36 homolog antibody
      • Zinc finger protein 36, C3H type, homolog (mouse) antibody
      • Zinc finger protein, C3H type, 36 homolog antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunocytochemistry/ Immunofluorescence - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunofluorescence analysis of transiently pCMV6-ENTRY ZFP36-transfected COS7 (african green monkey kidney fibroblast-like cell line) cells labeling TTP using ab124024 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunohistochemical analysis of paraffin-embedded human colon tissue staining TTP using ab124024 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/200 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : SV-T2 (Simian Virus 40-transformed BALB/c mouse embryo fibroblasts cell line) whole cell lysate
      Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
      Lane 4 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
      Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 6 : MDCK (canine kidney cell line) whole cell lysate
      Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

    • Flow Cytometry (Intracellular) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Flow Cytometry (Intracellular) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      HEK-293T cells transfected with either overexpressed plasmid (Red) or empty vector control plasmid (Blue) were immunostained by
      ab124024, and then analyzed by flow cytometry (Intracellular).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Western blot - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646) at 1/1000 dilution

      Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : pCMV6-ENTRY ZFP36-transfected HEK-293T cell lysate

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 34 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunohistochemical analysis of paraffin-embedded human adenocarcinoma colon tissue staining TTP using ab124024 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunohistochemical analysis of paraffin-embedded human ovary tissue staining TTP using ab124024 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining TTP using ab124024 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tristetraprolin/TTP antibody [OTI8B5] - BSA and Azide free (ab273646)

      Immunohistochemical analysis of paraffin-embedded human prostate tissue staining TTP using ab124024 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124024).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273646？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273646 尚未被引用在任何文献中。

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