Anti-TRIP13/PCH2抗体(ab128171)

Anti-TRIP13 antibody (ab128171) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab128171 was shown to bind specifically to TRIP13. A band was observed at 48 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TRIP13 knockout cell line. To generate this image, wild-type and TRIP13 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
a128171, at 1 µg/ml, staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128171 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 10 seconds.