Anti-TRIP13/PCH2抗体(ab128153)
Key features and details
- Rabbit polyclonal to TRIP13/PCH2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TRIP13/PCH2抗体
参阅全部 TRIP13/PCH2 一抗 -
描述
兔多克隆抗体to TRIP13/PCH2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum
-
免疫原
Synthetic peptide corresponding to Human TRIP13/PCH2 aa 1-50 (N terminal).
Database link: NP_004228.1 -
阳性对照
- HeLa, Jurkat and 293T whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab128153 was affinity purified using an epitope specific to TRIP13/PCH2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128153于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
序列相似性
Belongs to the AAA ATPase family. - Information by UniProt
-
数据库链接
- Entrez Gene: 609426 Dog
- Entrez Gene: 9319 Human
- Entrez Gene: 69716 Mouse
- Omim: 604507 Human
- SwissProt: E2R222 Dog
- SwissProt: Q15645 Human
- SwissProt: Q3UA06 Mouse
- SwissProt: D3K5L7 Pig
see all -
别名
- 16E1-BP antibody
- 16E1BP antibody
- Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody
see all
图片
-
Western blot - Anti-TRIP13/PCH2 antibody (ab128153)All lanes : Anti-TRIP13/PCH2 antibody (ab128153) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-TRIP13/PCH2 antibody (ab128153)Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
ab128153 at 1µg/ml staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128153 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab128153 被引用在 5 文献中.
- Vassel FM et al. REV7 Monomer Is Unable to Participate in Double Strand Break Repair and Translesion Synthesis but Suppresses Mitotic Errors. Int J Mol Sci 24:N/A (2023). PubMed: 37958783
- Liu L et al. KIF18B promotes breast cancer cell proliferation, migration and invasion by targeting TRIP13 and activating the Wnt/ß-catenin signaling pathway. Oncol Lett 23:112 (2022). PubMed: 35251343
- de Krijger I et al. MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair. Nat Commun 12:5421 (2021). PubMed: 34521823
- Hama T et al. DNA damage is overcome by TRIP13 overexpression during cisplatin nephrotoxicity. JCI Insight 6:N/A (2021). PubMed: 34806647
- Zhang Z et al. Bi-allelic Missense Pathogenic Variants in TRIP13 Cause Female Infertility Characterized by Oocyte Maturation Arrest. Am J Hum Genet 107:15-23 (2020). PubMed: 32473092