AB128187

Anti-TRIP13/PCH2抗体

Name: TRIP13/PCH2抗体 (ab128187)| Abcam中文官网
Brand: Abcam Limited
SKU: AB128187
Price: 4829 CNY
Availability: InStock

Anti-TRIP13/PCH2 antibody

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(1 Publication)

Rabbit Polyclonal TRIP13/PCH2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRIP13 aa 350 to C-terminus.

查看别名

PCH2, TRIP13, Pachytene checkpoint protein 2 homolog, Human papillomavirus type 16 E1 protein-binding protein, Thyroid hormone receptor interactor 13, Thyroid receptor-interacting protein 13, 16E1-BP, HPV16 E1 protein-binding protein, TR-interacting protein 13, TRIP-13

2 Images

Unknown

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (AB128187)

Detection of TRIP13/PCH2 by Western Blot of Immunprecipitate.
Lane 1 : ab128187 at 1μg/ml staining TRIP13/PCH2 in HeLa whole cell lysate immunoprecipitated using ab128187 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (ab128187)

Predicted band size: 49 kDa

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Unknown

Western blot - Anti-TRIP13/PCH2 antibody (AB128187)

All lanes:

Western blot - Anti-TRIP13/PCH2 antibody (ab128187) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human TRIP13 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15645

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRIP13 also known as PCH2 is a protein involved in the regulation of chromosome dynamics during meiosis and mitosis. It is approximately 48 kDa in size. TRIP13 plays an important role in promoting the disassembly of the synaptonemal complex and ensuring proper chromosome segregation. This protein is expressed in various tissues but shows higher levels in testis suggesting its importance in reproductive cell division.

Biological function summary

TRIP13 contributes to efficient cell cycle progression by participating in the spindle assembly checkpoint (SAC). It forms part of the mitotic checkpoint complex ensuring that chromosomes are correctly attached to the spindle microtubules before progression through mitosis. TRIP13 works in conjunction with other proteins like MAD2 and BUB1 to maintain genomic stability.

Pathways

TRIP13 is involved in the cell cycle control and DNA repair pathways. It influences the mitotic spindle checkpoint interacting with proteins such as MAD2 which ensures that cells do not proceed to anaphase until all chromosomes reach proper alignment. TRIP13 also plays a role in homologous recombination repair by regulating the processing of recombination intermediates.

TRIP13 has been implicated in cancer and infertility. Its overexpression or mutation has been linked to tumor progression and resistance to certain chemotherapeutic agents due to its role in chromosome segregation and genomic stability. Additionally TRIP13 dysfunction may contribute to infertility through its impact on meiosis with potential interactions with proteins involved in reproductive processes such as SYCP3.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double-strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity). Plays a role in mitotic spindle assembly checkpoint (SAC) activation (PubMed : 28553959).

See full target information TRIP13

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:3211-3218 PubMed30867751

2019

MicroRNA-218 inhibits the cell proliferation and migration in clear cell renal cell carcinoma through targeting cancerous inhibitor of protein phosphatase 2A.

Applications

Unspecified application

Species

Unspecified reactive species

Ruojing Wei,Xiongjun Ye,Yawei Zhao,Ning Jia,Tongwei Liu,Wenfeng Lian,Hongjian Wei,Gang Zhang,Lijie Song

View all publications

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Anti-TRIP13/PCH2抗体

Anti-TRIP13/PCH2 antibody

Immunoprecipitation - Anti-TRIP13/PCH2 antibody (AB128187)

Western blot - Anti-TRIP13/PCH2 antibody (AB128187)