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AB194365

Anti-TRIO抗体

Anti-TRIO antibody

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(3 Publications)

Mouse Polyclonal TRIO antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIO aa 1950 to C-terminus.

查看别名

Triple functional domain protein, PTPRF-interacting protein, TRIO

2 Images
Western blot - Anti-TRIO antibody (AB194365)
  • WB

Supplier Data

Western blot - Anti-TRIO antibody (AB194365)

Predicted MWt of immunogen : 38.21

All lanes:

Western blot - Anti-TRIO antibody (ab194365) at 1/1000 dilution

All lanes:

recombinant Human TRIO protein (immunogen) at 0.2 µg

Predicted band size: 347 kDa,73 kDa

false

Western blot - Anti-TRIO antibody (AB194365)
  • WB

Supplier Data

Western blot - Anti-TRIO antibody (AB194365)

All lanes:

Western blot - Anti-TRIO antibody (ab194365) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 347 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

Recombinant Fragment Protein within Human TRIO aa 1950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75962

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯度
Whole antiserum
存储溶液
Constituents: 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRIO also known as Triple Functional Domain Protein or Trio Rho guanine nucleotide exchange factor acts mechanically as a guanine nucleotide exchange factor. It facilitates the activation of the Rho family of small GTPases specifically Rac1 and RhoG. TRIO weighs approximately 349 kDa and is widely expressed in different tissues especially in the central nervous system. This protein modulates signal transduction associated with cytoskeletal dynamics cell migration and neuronal development.
Biological function summary

TRIO influences cellular processes like actin cytoskeleton organization and axonal outgrowth. It serves as part of multi-protein complexes that guide the intracellular signaling cascades. Particularly TRIO plays a central role in developmental pathways by promoting neuronal growth and differentiation through Rac1 activation. Its function is important for establishing the architecture of the nervous system.

Pathways

TRIO participates in key signal transduction pathways such as the Rho GTPase cycle and the axon guidance pathway. It acts in concert with other proteins like Rac1 and RhoG to regulate cell adhesion and neuronal pathfinding. Through these pathways TRIO contributes to the polarization and directional movement of cells which is necessary for proper tissue and organ development.

TRIO connects to several neurological conditions including intellectual disability and schizophrenia. Mutations in the TRIO gene as well as dysregulation of its related proteins can disrupt normal brain development and function. Particularly alterations in Rac1 and RhoG signaling have been implicated in these disorders linking TRIO to their pathogenesis and potential therapeutic targets.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Guanine nucleotide exchange factor (GEF) for RHOA and RAC1 GTPases (PubMed : 22155786, PubMed : 27418539, PubMed : 8643598). Involved in coordinating actin remodeling, which is necessary for cell migration and growth (PubMed : 10341202, PubMed : 22155786). Plays a key role in the regulation of neurite outgrowth and lamellipodia formation (PubMed : 32109419). In developing hippocampal neurons, limits dendrite formation, without affecting the establishment of axon polarity. Once dendrites are formed, involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors (AMPARs) at CA1 excitatory synapses (By similarity). May act as a regulator of adipogenesis (By similarity).
See full target information TRIO
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Current biology : CB 32:1577-1592.e8 PubMed35290799

2022

Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Tak Shun Fung,Rajarshi Chakrabarti,Jana Kollasser,Klemens Rottner,Theresia E B Stradal,Frieda Kage,Henry N Higgs

Cell death & disease 12:1148 PubMed34893584

2021

Rho-GEF Trio regulates osteosarcoma progression and osteogenic differentiation through Rac1 and RhoA.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Wang,Lichan Yuan,Xiaohong Xu,Zhongyin Zhang,Yuhuan Ma,Leilei Hong,Junqing Ma

Cell reports 29:2944-2952.e5 PubMed31801062

2019

Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremiah D Paskus,Chen Tian,Erin Fingleton,Christine Shen,Xiaobing Chen,Yan Li,Samuel A Myers,John D Badger,Michael A Bemben,Bruce E Herring,Katherine W Roche
View all publications
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