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AB312833

重组Anti-TRIM67抗体[EPR25994-6] - BSA and Azide free

Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRI67 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples.

查看别名

TNL, TRIM67, Tripartite motif-containing protein 67, TRIM9-like protein

2 Images
Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)
  • WB

Supplier Data

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

This data was developed using ab312832, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : lung (PMID : 31420404), liver (PMID : 31420404). The bands beneath the target band (83kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-TRIM67 antibody [EPR25994-6] (<a href='/products/primary-antibodies/trim67-antibody-epr25994-6-ab312832'>ab312832</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 60 µg

Lane 2:

Human lung tissue lysate at 60 µg

Lane 3:

human liver tissue lysate at 60 µg

Lane 4:

Mouse cerebellum tissue lysate at 60 µg

Lane 5:

Mouse lung tissue lysate at 60 µg

Lane 6:

Mouse liver tissue lysate at 60 µg

Lane 7:

Rat cerebellum tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 60 µg

Lane 9:

Rat liver tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 83 kDa

false

Exposure time: 15s

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)
  • WB

Supplier Data

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

This data was developed using ab312832, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-TRIM67 antibody [EPR25994-6] (<a href='/products/primary-antibodies/trim67-antibody-epr25994-6-ab312832'>ab312832</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg

Lane 2:

293T transfected with a TRIM67 expression vector containi a His tag whole cell lysate at 20 µg

Lane 3:

293T transfected with a TRIM9 expression vector containi a His tag whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 3s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25994-6

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: 100% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRIM67 also known as Tripartite Motif Containing 67 is a protein encoded by the TRIM67 gene. It belongs to the tripartite motif (TRIM) family of proteins characterized by an N-terminal RING finger domain one or two B-box motifs and a coiled-coil region. TRIM67 has a molecular mass of approximately 53 kDa. This protein is mainly expressed in the brain particularly in neurons highlighting its importance in neural tissues.
Biological function summary

TRIM67 plays an important role in neuronal development and differentiation. It contributes to processes like axon outgrowth and guidance which are critical for proper neuronal network formation. TRIM67 does not function as part of a complex but interacts with other proteins for executing its roles. It is known to modulate several signaling pathways key to cell proliferation and survival emphasizing its importance in cellular maintenance and integrity.

Pathways

TRIM67 impacts the regulation of the Wnt signaling pathway which is essential for numerous developmental processes. Moreover it has a relationship with the PI3K/AKT signaling pathway which is important for cell growth and apoptosis. In these pathways TRIM67 can interact with proteins like DVL and AKT1 contributing to their regulation and overall pathway stability. This connectivity underlines the multifaceted role TRIM67 plays in cell signaling and developmental biology.

TRIM67 has been associated with neurological disorders given its strong expression in neuronal tissues. Specifically it relates to conditions like autism spectrum disorders (ASD) where disruptions in its expression or mutations could impact neural connectivity. Additionally neurodegenerative diseases such as Alzheimer's disease may also involve TRIM67 due to its role in neuronal survival and function. These associations highlight a connection with proteins like APP and BDNF which are linked to neural health and disease.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information TRIM67
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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关联产品

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Alternative Version
Primary Antibodies

AB312832

Anti-TRIM67 antibody [EPR25994-6]

primary-antibodies

trim67-antibody-epr25994-6-ab312832

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