Anti-TRIM36抗体(ab272672)
Key features and details
- Rabbit polyclonal to TRIM36
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM36抗体 -
描述
兔多克隆抗体to TRIM36 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human TRIM36 aa 200-350.
Database link: Q9NQ86 -
阳性对照
- WB: RT4, U-251 MG cells. IF: RH-30 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization. -
组织特异性
Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart. -
序列相似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger. -
细胞定位
Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm. - Information by UniProt
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数据库链接
- Entrez Gene: 55521 Human
- GenBank: NM_018700 Human
- Omim: 609317 Human
- SwissProt: Q9NQ86 Human
- Unigene: 519514 Human
- Unigene: 638953 Human
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别名
- Acrosome RBCC protein antibody
- E3 ubiquitin protein ligase TRIM36 antibody
- E3 ubiquitin-protein ligase Trim36 antibody
see all
图片
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Western blot - Anti-TRIM36 antibody (ab272672)All lanes : Anti-TRIM36 antibody (ab272672) at 0.4 µg/ml
Lane 1 : RT4 cells (Human urinary bladder cancer cell line)
Lane 2 : U-251 MG cells (Human brain glioma cell line) -
Immunocytochemistry/ Immunofluorescence - Anti-TRIM36 antibody (ab272672)PFA fixed, Triton X-100 permeablized RH-30 cells (human bone marrow metastasis cell line) labeling TRIM36 (green) using ab272672 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272672 被引用在 1 文献中.
- Yan H et al. Extracellular Polysaccharide from Rhizopus nigricans Inhibits Hepatocellular Carcinoma via miR-494-3p/TRIM36 Axis and Cyclin E Ubiquitination. J Clin Transl Hepatol 10:608-619 (2022). PubMed: 36062277