AB96612

Anti-TRIM32 抗体

Name: TRIM32抗体 (ab96612)| Abcam中文官网
Brand: Abcam Limited
SKU: AB96612
Price: 4795 CNY
Availability: InStock

Anti-TRIM32 antibody

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(12 Publications)

Rabbit Polyclonal TRIM32 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Transfected cell lysate - Human, Human samples. Cited in 12 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM32 aa 1-300.

查看别名

HT2A, TRIM32, E3 ubiquitin-protein ligase TRIM32, 72 kDa Tat-interacting protein, RING-type E3 ubiquitin transferase TRIM32, Tripartite motif-containing protein 32, Zinc finger protein HT2A

2 Images

Supplier Data

Western blot - Anti-TRIM32 antibody (AB96612)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-TRIM32 antibody (ab96612) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-TRIM32 antibody (AB96612)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-TRIM32 antibody (ab96612) at 1/20000 dilution

Lane 1:

293T whole cell lysate at 5 µg

Lane 2:

DDDDK-human TRIM32-transfected 293T whole cell lysate extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 72 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human TRIM32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q13049

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

TRIM32 also known as Tripartite Motif Containing 32 is a protein that functions as an E3 ubiquitin ligase. It plays a role in attaching ubiquitin molecules to proteins tagging them for degradation by the proteasome. TRIM32 consists of a RING domain a B-box and a coiled-coil region which are characteristic of the TRIM family. The molecular mass of TRIM32 is approximately 71 kDa. It is expressed in various tissues with notable expression in skeletal muscle and the heart implying an important role in muscle physiology.

Biological function summary

TRIM32 participates in regulating muscle cell differentiation and proliferation. It interacts with several proteins including dysbindin and myospryn suggesting its involvement in a multi-protein complex. TRIM32 has a regulatory role in muscle maintenance where it influences the stability and function of muscle proteins. Moreover it is also involved in neurogenesis affecting neuronal differentiation and survival emphasizing its role beyond muscle tissues.

Pathways

TRIM32 plays a significant role in the PI3K/Akt signaling pathway and the TGF-beta signaling pathway. In the PI3K/Akt pathway TRIM32 modulates cell growth and survival interacting with proteins such as Akt and mTOR. In the context of TGF-beta signaling TRIM32 can influence cellular processes like proliferation and differentiation possibly by interacting with SMAD proteins. These interactions indicate its importance in cellular growth and differentiation pathways.

TRIM32 is linked with limb-girdle muscular dystrophy type 2H and certain forms of sarcopenia. Mutations in TRIM32 cause dysfunction in muscle protein turnover leading to muscle deterioration and weakness. In limb-girdle muscular dystrophy TRIM32 mutations disrupt its interaction with dysbindin impairing muscle structure. Furthermore TRIM32 might be involved in neurodegenerative diseases with connections to proteins like alpha-synuclein hinting at a potential role in Parkinson's disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy (PubMed : 19349376, PubMed : 31123703). Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1 (PubMed : 22745133). Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages (PubMed : 37543647). Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner (PubMed : 28898289). Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions (PubMed : 32918979). Ubiquitinates XIAP and targets it for proteasomal degradation (PubMed : 21628460). Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation (PubMed : 19349376). May ubiquitinate BBS2 (PubMed : 22500027). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1 : interaction with AMBRA1 is required for ULK1 activation (PubMed : 31123703). Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL (PubMed : 34888944). Under metabolic stress and phosphorylation by CHK2, mediates 'Lys-63'-linked ubiquitination of ATG7 at 'Lys-45' to initiate autophagy (PubMed : 37943659).. (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo (PubMed : 7778269). Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo (PubMed : 7778269).

See full target information TRIM32

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:31037 PubMed40849525

2025

Proteomics on choroidal neovascularization based on itraq and the protective effect of TAB1 in CNV.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Du,Shaoqiu Jiang,Lujia Feng,Jing Lu,Hui Peng,Xiyuan Zhou

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70118 PubMed39439252

2024

m6A demethylase FTO transcriptionally activated by SP1 improves ischemia reperfusion-triggered acute kidney injury by activating Ambra1/ULK1-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Yuanfei Liu,Weiping Tu,Yanxia Chen,Chengyun Xu,Chong Huang

Journal of virology 98:e0000524 PubMed38717113

2024

Degradation of TRIM32 is induced by RTA for Kaposi's sarcoma-associated herpesvirus lytic replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Zhang,Zhongwei Dong,Feng Gu,Yifei Xu,Ying Li,Wen Sun,Wutian Rao,Shujuan Du,Caixia Zhu,Yuyan Wang,Fang Wei,Qiliang Cai

Cell & bioscience 12:183 PubMed36371321

2022

mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo Tang

PLoS biology 19:e3001432 PubMed34813590

2021

Homeostatic scaling is driven by a translation-dependent degradation axis that recruits miRISC remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Balakumar Srinivasan,Sarbani Samaddar,Sivaram V S Mylavarapu,James P Clement,Sourav Banerjee

Scandinavian journal of immunology 91:e12876 PubMed32145086

2020

TRIM32 promotes inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liang,Min Song,Kewu Xu,Chenglong Guo,Hongbin Xu,Hongwei Zhang,Lanping Xu

Cell reports 30:98-111.e5 PubMed31914402

2020

TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyu Luo,Xiaowei Wu,Yabing Nan,Wan Chang,Pengfei Zhao,Yiping Zhang,Dan Su,Zhihua Liu

Genome biology 20:262 PubMed31791371

2019

eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR.

Applications

Unspecified application

Species

Unspecified reactive species

Ania Wilczynska,Sarah L Gillen,Tobias Schmidt,Hedda A Meijer,Rebekah Jukes-Jones,Claudia Langlais,Kari Kopra,Wei-Ting Lu,Jack D Godfrey,Benjamin R Hawley,Kelly Hodge,Sara Zanivan,Kelvin Cain,John Le Quesne,Martin Bushell

Cells 8: PubMed30884854

2019

Analysis of the Zn-Binding Domains of TRIM32, the E3 Ubiquitin Ligase Mutated in Limb Girdle Muscular Dystrophy 2H.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Lazzari,Medhat S El-Halawany,Matteo De March,Floriana Valentino,Francesco Cantatore,Chiara Migliore,Silvia Onesti,Germana Meroni

Oncology letters 13:3131-3138 PubMed28521418

2017

Overexpression of E3 ubiquitin ligase tripartite motif 32 correlates with a poor prognosis in patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiro Ito,Kazuhiro Migita,Sohei Matsumoto,Kohei Wakatsuki,Tetsuya Tanaka,Tomohiro Kunishige,Hiroshi Nakade,Mitsuhiro Nakatani,Yoshiyuki Nakajima

View all publications

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Anti-TRIM32 抗体

Anti-TRIM32 antibody

Western blot - Anti-TRIM32 antibody (AB96612)

Western blot - Anti-TRIM32 antibody (AB96612)