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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase

Anti-TRIM2抗体(ab3942)

  • Datasheet
Submit a review Submit a question References (2)

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Western blot - Anti-TRIM2 antibody (ab3942)

    Key features and details

    • Goat polyclonal to TRIM2
    • Suitable for: IHC-P, WB
    • Reacts with: Rat, Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-TRIM2抗体
    • 描述

      山羊多克隆抗体to TRIM2
    • 宿主

      Goat
    • 经测试应用

      适用于: IHC-P, WBmore details
    • 种属反应性

      与反应: Rat, Human
      预测可用于: Cow, Dog
    • 免疫原

      Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
      Sequence:

      DSGNHCFKVYRYLQ

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • RING Finger E3 Ligase
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • Other

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3942于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.5 µg/ml.

    A 1 hour primary incubation is recommended for this product.

    说明
    IHC-P
    Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    WB
    Use a concentration of 0.5 µg/ml.

    A 1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      May contribute to the alteration of neural cellular mechanisms.
    • 序列相似性

      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 filamin repeat.
      Contains 6 NHL repeats.
      Contains 1 RING-type zinc finger.
    • 结构域

      The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
    • 细胞定位

      Cytoplasm.
    • Target information above from: UniProt accession Q9C040 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 23321 Human
      • Entrez Gene: 361970 Rat
      • Omim: 614141 Human
      • SwissProt: Q9C040 Human
      • SwissProt: D3ZQG6 Rat
      • Unigene: 435711 Human
      • 别名

        • E3 ubiquitin protein ligase TRIM2 antibody
        • KIAA0517 antibody
        • RING finger protein 86 antibody
        • RNF86 antibody
        • Trim2 antibody
        • TRIM2_HUMAN antibody
        • Tripartite motif containing 2 antibody
        • Tripartite motif protein 2 antibody
        • Tripartite motif protein TRIM2 antibody
        • Tripartite motif-containing 2 antibody
        • Tripartite motif-containing protein 2 antibody
        see all

      图片

      • Western blot - Anti-TRIM2 antibody (ab3942)
        Western blot - Anti-TRIM2 antibody (ab3942)
        Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg

        Observed band size: 80,85 kDa why is the actual band size different from the predicted?
        Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.



        Primary incubation was for 1 hour. Detected by chemiluminescence.

      实验方案

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet download

        Download

      文献 (2)

      发表研究结果有使用 ab3942?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3942 被引用在 2 文献中.

      • Yi H  et al. Knockdown of long non-coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA-369-3p/TRIM2 axis. Int J Mol Med 47:N/A (2021). PubMed: 33760118
      • Qin Y  et al. TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma. Int J Oncol 53:1643-1656 (2018). PubMed: 30066883

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