Anti-TRIM11抗体(ab111694)
Key features and details
- Rabbit polyclonal to TRIM11
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TRIM11抗体
参阅全部 TRIM11 一抗 -
描述
兔多克隆抗体to TRIM11 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
-
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-155 of Human TRIM11.
-
阳性对照
- 293T, A431, Jurkat and Raji whole cell lysates; Human Hepatoma tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111694于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
|
| 说明 |
|---|
|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
靶标
-
功能
E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle. -
组织特异性
Ubiquitous. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. -
结构域
The coiled-coil domain and the B30.2 domain are both necessary for interaction with HN and PAX6 (By similarity). They are also involved in MED15-binding.
The B30.2 domain may be involved cellular protein quality control by promoting the degradation of insoluble ubiquitinated proteins. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 81559 Human
- Entrez Gene: 338364 Mouse
- Entrez Gene: 94091 Mouse
- Entrez Gene: 360534 Rat
- Omim: 607868 Human
- SwissProt: Q96F44 Human
- SwissProt: Q99PQ2 Mouse
- SwissProt: B1H278 Rat
see all -
别名
- BIA 1 antibody
- BIA1 antibody
- E3 ubiquitin protein ligase TRIM11 antibody
see all
图片
-
Western blot - Anti-TRIM11 antibody (ab111694)Anti-TRIM11 antibody (ab111694) at 1/2000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 53 kDa
7.5% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM11 antibody (ab111694)ab111694 at 1/500 dilution staining TRIM11 in Formalin-fixed, paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
数据表及文件
-
SDS download
-
Datasheet download
文献 (13)
ab111694 被引用在 13 文献中.
- Zhang P et al. TRIM11 regulated by m6A modification promotes the progression of cervical cancer by PHLPP1 ubiquitination. Neoplasma 70:659-669 (2023). PubMed: 38053376
- Pan Y et al. TRIM11 Posttranscriptionally Modulated by miR-5193 Facilitates Tumor Growth and Metastasis of Prostate Cancer. Technol Cancer Res Treat 22:15330338231178639 (2023). PubMed: 37248611
- He F et al. Downregulation of tripartite motif protein 11 attenuates cardiomyocyte apoptosis after ischemia/reperfusion injury via DUSP1-JNK1/2. Cell Biol Int 46:148-157 (2022). PubMed: 34694031
- Zhou L et al. The E3 Ubiquitin Ligase TRIM11 Facilitates Gastric Cancer Progression by Activating the Wnt/β-Catenin Pathway via Destabilizing Axin1 Protein. J Oncol 2022:8264059 (2022). PubMed: 35237324
- Xiao Q et al. Regulation of KDM5C stability and enhancer reprogramming in breast cancer. Cell Death Dis 13:843 (2022). PubMed: 36192394