重组Anti-TRIF (TICAM1)抗体[EPR24995-152] (ab302562)
![Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)](https://www.abcam.cn/ps/products/302/ab302562/Images/ab302562-522379-anti-trif-ticam1-antibody-epr24995-152-western-blot-wildtype-u87-mg-ticam1-knockout-u87-mg-raji-hl60.jpg "Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24995-152] to TRIF
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRIF (TICAM1)抗体[EPR24995-152]
参阅全部 TRIF 一抗 -
描述
兔单克隆抗体[EPR24995-152] to TRIF -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt (Intra),ICC/IF or IHC-P -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Raji, Ramos, HL-60, HeLa and U-87 MG cell lysate. IP: Raji cell lysate.
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常规说明
ab302562 does not react in: WB with mouse and rat samples; IHC-P with human, mouse, and rat specimens; ICC/IF and intracellular flow cytometry with human tissues.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24995-152 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab302562于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 76 kDa).
|
| 说明 |
|---|
|
IP
1/30. |
|
WB
1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 76 kDa). |
靶标
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功能
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. -
组织特异性
Ubiquitously expressed but with higher levels in liver. -
序列相似性
Contains 1 TIR domain. -
结构域
The N-terminal region is essential for activation of the IFNB promoter activity. -
翻译后修饰
Phosphorylated by TBK1. - Information by UniProt
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数据库链接
- Entrez Gene: 148022 Human
- Omim: 607601 Human
- SwissProt: Q8IUC6 Human
- Unigene: 29344 Human
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别名
- IIAE6 antibody
- MGC35334 antibody
- MyD88 3 antibody
see all
图片
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Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562)All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution
Lane 1 : Wild-type U-87 MG cell lysate
Lane 2 : TICAM1 knockout U-87 MG cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Western blot: Anti-TICAM1 antibody [EPR24995-152] (ab302562) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab302562 was shown to bind specifically to TICAM1. A band was observed at 85 kDa in wild-type U-87 MG cell lysates with no signal observed at this size in TICAM1 knockout cell line. To generate this image, wild-type and TICAM1 knockout U-87 MG cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)](https://www.abcam.cn/ps/products/302/ab302562/Images/AB302562-490679-anti-trif-ticam1-antibody-western-blot-raji.jpg)
Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma b lymphocyte) whole cell lysate
Lane 2 : HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Performed under non-reducing conditions.
Predicted band size: 76 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsDiluting and blocking buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Negative control: HL-60.
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![Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)](https://www.abcam.cn/ps/products/302/ab302562/Images/AB302562-490678-anti-trif-ticam1-antibody-western-blot-ramos-hela.jpg)
Western blot - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)All lanes : Anti-TRIF (TICAM1) antibody [EPR24995-152] (ab302562) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma b lymphocyte) whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under non-reducing conditions.
Predicted band size: 76 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesDiluting and blocking buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The identity of the lower MW band at approximately 18 kDa is unknown. -
![Immunoprecipitation - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)](https://www.abcam.cn/ps/products/302/ab302562/Images/AB302562-2-anti-trif-ticam1-antibody-epr24995-152-immunoprecipitation-raji.jpg)
Immunoprecipitation - Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)TRIF (TICAM1) was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma b lymphocyte) whole cell lysate with ab302562 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302562 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate 10 µg (Input)
Lane 2: ab302562 IP in Raji whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302562 in Raji whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
Observed MW (kDa): 98.
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![Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)](https://www.abcam.cn/ps/products/302/ab302562/Images/AB302562-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-TRIF (TICAM1) antibody [EPR24995-152] (AB302562)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab302562 被引用在 1 文献中.
- Yue L et al. Toll-like receptor 4 promotes the inflammatory response in septic acute kidney injury by promoting p38 mitogen-activated protein kinase phosphorylation. J Bioenerg Biomembr 55:353-363 (2023). PubMed: 37605037