Anti-Trichohyalin抗体[AE15] (ab58755)
![Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)](https://www.abcam.cn/ps/products/58/ab58755/Images/Trichohyalin-Primary-antibodies-ab58755-2.jpg "Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)")
Key features and details
- Mouse monoclonal [AE15] to Trichohyalin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Trichohyalin抗体[AE15]
参阅全部 Trichohyalin 一抗 -
描述
小鼠单克隆抗体[AE15] to Trichohyalin -
宿主
Mouse -
特异性
This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation. -
经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Tissue, cells or virus corresponding to Human Trichohyalin.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
AE15 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab58755于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 220 kDa.
|
|
| IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 19038788
|
| IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 19786578
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 220 kDa. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 19038788 |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 19786578 |
靶标
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功能
Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation. -
组织特异性
Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium. -
序列相似性
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains. -
发展阶段
Expressed during late differentiation of the epidermis. -
结构域
Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species. -
翻译后修饰
Substrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase. - Information by UniProt
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数据库链接
- Entrez Gene: 7062 Human
- Entrez Gene: 99681 Mouse
- SwissProt: Q07283 Human
- Unigene: 432416 Human
- Unigene: 553300 Human
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别名
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
see all
图片
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Western blot - Anti-Trichohyalin antibody [AE15] (ab58755)Anti-Trichohyalin antibody [AE15] (ab58755) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 220 kDa
Observed band size: 220 kDa
Additional bands at: 165 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab58755 被引用在 17 文献中.
- Shi TW et al. Effects of NCSTN Mutation on Hair Follicle Components in Mice. Dermatology 239:60-71 (2023). PubMed: 35843211
- Makino T et al. Altered expression of S100 fused-type proteins in an atopic dermatitis skin model. Exp Dermatol 32:2160-2165 (2023). PubMed: 36995036
- Leng X et al. Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay". Exp Dermatol N/A:N/A (2018). PubMed: 30033549
- Tripurani SK et al. Suppression of Wnt/ß-catenin signaling by EGF receptor is required for hair follicle development. Mol Biol Cell 29:2784-2799 (2018). PubMed: 30188763
- Sardella C et al. Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion. J Invest Dermatol 138:500-510 (2018). PubMed: 28964716