Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证重组RabMAb

重组Anti-TRIB3抗体[EPR3151Y] (ab75846)

Reviews (1)Q&A (1)References (18)
Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
  • Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
  • Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
  • Anti-TRIB3 antibody [EPR3151Y] (ab75846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3151Y] to TRIB3
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-TRIB3抗体[EPR3151Y]
    参阅全部 TRIB3 一抗

  • 描述

    兔单克隆抗体[EPR3151Y] to TRIB3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HCT116 cell lysates; 293T cell lysates, untreated and treated with Ionomycin and TPA. IP: 293T.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75846于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP
1/10.
说明
WB
1/5000 - 1/20000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP
1/10.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

靶标

  • 功能

    Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.

  • 组织特异性

    Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.

  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
    Contains 1 protein kinase domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • C20orf97 antibody
      • Neuronal cell death inducible putative kinase antibody
      • Neuronal cell death-inducible putative kinase antibody
      • NIPK antibody
      • p65 interacting inhibitor of NF-kappaB antibody
      • p65-interacting inhibitor of NF-kappa-B antibody
      • SINK antibody
      • SKIP 3 antibody
      • SKIP3 antibody
      • TRB 3 antibody
      • TRB-3 antibody
      • TRB3 antibody
      • TRIB 3 antibody
      • Trib3 antibody
      • TRIB3_HUMAN antibody
      • Tribbles homolog 3 antibody
      • Tribbles pseudokinase 3 antibody
      • Tribbles3 antibody
      see all

    图片

    • Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

      Lane 1 : Wild-type HCT116 cell lysate
      Lane 2 : TRIB3 knockout HCT116 cell lysate
      Lane 3 : Hap1 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab75846 observed at 40 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab75846 was shown to react with TRIB3 in HCT 116 wild-type cells in western blot with loss of signal observed in TRIB3 knockout cell line ab273718 (TRIB3 knockout cell lysate ab275249). HCT 116 wild-type and TRIB3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75846 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      All lanes : Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

      Lane 1 : 293T cell lysates, treated with Ionomycin and
      TPA
      Lane 2 : 293T cell lysates, untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (ab75846)

      TRIB3 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg with 75846 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 µg

      Lane 2: ab75846 IP in 293T whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75846 in 293T whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-TRIB3 antibody [EPR3151Y] (ab75846)
      Anti-TRIB3 antibody [EPR3151Y] (ab75846)

    文献 (18)

    发表研究结果有使用 ab75846？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75846 被引用在 18 文献中.

    • Zhang Q  et al. Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma. Front Immunol 14:1324205 (2023). PubMed: 38090559
    • Zhou W  et al. Deletion of TRIB3 disrupts the tumor progression induced by integrin αvβ3 in lung cancer. BMC Cancer 22:459 (2022). PubMed: 35473511
    • Cordova RA  et al. GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis. Elife 11:N/A (2022). PubMed: 36107759
    • Wang RQ  et al. Tribbles pseudokinase 3 (TRIB3) contributes to the progression of hepatocellular carcinoma by activating the mitogen-activated protein kinase pathway. Ann Transl Med 9:1253 (2021). PubMed: 34532390
    • Orea-Soufi A  et al. The Pseudokinase TRIB3 Negatively Regulates the HER2 Receptor Pathway and Is a Biomarker of Good Prognosis in Luminal Breast Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34771470
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-TRIB3 antibody [EPR3151Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human cell lines)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    35 µg
    Specification
    human cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 31 2019

    Question

    Hello, I would like some informations about those antibodies against TRB3 : ab50516 (Aminoterminal) and ab75846 I would like to immunoprecipitate TRB3 and measure AKT in order to study their interaction in C2C12 cells. What is the difference between those antibodies ? Is there a better one for the co-immunoprecipitation ? What is the target (sequence) of those antibodies ? Are they working stricly with human ? Are they working with mice ? Thanks for your help.

    Read More
    Answer

    Thank you for contacting us.  Both of these anti-TRIB3 antibodies has been tested for immunoprecipiation and are guaranteed for its use in this application. They have not been tested side by side and so we have no data on whether one may work better for Co-IP than the other. The immunogen sequence for ab50516 is GSLSRKKRLELDDNLDTER and is aa 11-29. The immunogen sequence for ab75846 is considered propreitary and cannot be given out, although it is a small peptide from the C-terminus.  Both antibodies are tested and guaranteed in human. ab50516 has not been tested in mouse. ab75846 has been tested in mouse and DOES NOT react. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com